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TOYOPEARL SuperQ-650C

TOYOPEARL SuperQ-650C resin is a strong anion exchanger for biomolecule purification. This resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with quaternary amine (Q) strong anion exchange groups.  This resin has a 100 nm pore size and a 100 µm bead size.

TOYOPEARL SuperQ-650C resin is best used for high throughput capture, intermediate purification, and polishing process steps. If greater resolution is needed, the smaller particle size TOYOPEARL SuperQ-650M, TOYOPEARL SuperQ-650S or TSKgel SuperQ-5PW resins may be used. As both the TOYOPEARL SuperQ-650 and TSKgel SuperQ-5PW resins use the same ligand and backbone chemistry, the selectivity between the two resin types will be unchanged. 

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
43275TOYOPEARL SuperQ-650C100 mL100  263
17231TOYOPEARL SuperQ-650C250 mL100  606
17232TOYOPEARL SuperQ-650C1 L100  -
17233TOYOPEARL SuperQ-650C5 L100  -
                 

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 100 µm
Ligand type: strong anion
Ligand pKa: 12.2
DBC: 105 - 155 g/L
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years

Structure:

IEX_SuperQ-650C-M-S_Structure.png

 
 
 
 

    

Scale Up or Down using the Same Ligand


IEX_SuperQ-5PW-Fig1.png

Resins: A. TOYOPEARL SuperQ-650C, 100 µm
B. TOYOPEARL SuperQ-650M, 65 µm
C. TOYOPEARL SuperQ-650S, 35 µm
D. TSKgel SuperQ-5PW (30), 30 µm
E. TSKgel SuperQ-5PW (20), 20 µm
F. TSKgel SuperQ-5PW, 10 µm
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 0.02 mol/L Tris-HCl, pH 8.5
Buffer B: 0.5 mol/L NaCl in buffer A
Gradient: 60 min linear gradient from buffer A to buffer B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Sample: mAb in mouse ascites (dilution, × 5)
Load volume: 100 µL 


Code #DescriptionLiterature Type
AN024 Preserving Chromatographic Selectivity While Improving Your Process PurificationApplication Note
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
RIS0029 TOYOPEARL SuperQ-650Resin Information Sheet
P/NDescriptionPrice ($) 
OC41MDAEX-96Resin Seeker TOYOPEARL AIEX 96-well plate kit384