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TOYOPEARL DEAE-650M

TOYOPEARL DEAE-650M resin is a weak anion exchanger for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with diethylaminoethyl (DEAE) weak anion exchange groups.  TOYOPEARL DEAE-650M has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates.

TOYOPEARL DEAE-650M has a 100 nm pore size and a 65 µm bead size and can be used for high throughput intermediate purification and polishing process steps. If greater resolution is needed, the smaller particle TOYOPEARL DEAE-650S or the TSKgel DEAE-5PW resins may be used. As both the TOYOPEARL DEAE-650 and TSKgel DEAE-5PW resins use the same ligand and backbone chemistry, the selectivity between the two resins will be unchanged. 

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
43201TOYOPEARL DEAE-650M100 mL65  198
07473TOYOPEARL DEAE-650M250 mL65  454
14693TOYOPEARL DEAE-650M1 L65  -
07974TOYOPEARL DEAE-650M5 L65  -
18367TOYOPEARL DEAE-650M50 L65  -
               

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 65 µm
Ligand type: weak anion
Ligand pKa: 11.5
DBC: 25 - 35 g/L
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13

Structure:

IEX_DEAE-650C-M-S_Structure.png

 
 
 

           

Blood Proteins

IEX_DEAE-650-Fig38A.png


Resins: 1. TOYOPEARL DEAE-650S
2. TOYOPEARL DEAE-650M
Column size: 16 mm ID × 15 cm
Mobile phase: Buffer A: 50 mmol/L Tris-HCl, pH 8.6
Buffer B:50 mmol/L Tris-HCl, pH 8.6 + 0.5 mol/L NaCl
Gradient: linear gradient from buffer A to buffer B in 200 mL total volume
Flow Rate: 45 cm/hr (1.5 mL/min) 
Detection: UV @ 280 nm
Temperature: 25 °C
Sample: human serum

IEX_DEAE-650-Fig38B.png

Column: TSKgel G3000SW, 7.5 mm ID × 30 cm × 2 in series
Mobile phase: 0.1 mol/L phosphate, pH 6.8 + 0.1 mol/L sodium sulfate
Detection: UV @ 280 nm
Temperature: 25 °C
Samples: 1. crude human serum
2. albumin fraction from TOYOPEARL DEAE-650S
3. albumin fraction from TOYOPEARL DEAE-650M

Kato, Y.; Nakamura, K.; Hashimoto, T. Characterization of TSK-GEL DEAE-Toyopearl 650 Ion Exchanger. J. Chromatogr. 1982, 245, 193-211. 

For additional applications, please see our applications database.

 
           

Dynamic Binding Capacity Comparision of AIEX Resins for BSA @ 100 cm/hr

IEX_SuperQ_DEAE_QAE-650M_DBC_rev.png

 

Resins: binding capacity
(10% breakthrough)
TOYOPEARL DEAE-650M 40.1 g/L
TOYOPEARL QAE-650M
81.3 g/L
TOYOPEARL SuperQ-650M
128.8 g/L
    

Scale Up or Down using the Same Ligand


IEX_DEAE-5PW_selectivity.png

Resins: TOYOPEARL DEAE-650C, 100µm
TOYOPEARL DEAE-650M, 65 µm
TOYOPEARL DEAE-650S, 35 µm
TSKgel DEAE-5PW (30), 30µm
TSKgel DEAE-5PW (20), 20µm
TSKgel DEAE-5PW, 10µm
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 0.02 mol/L Tris-HCl, pH 8.5
Buffer B: 0.5 mol/L NaCl in buffer A
Gradient: 60 min linear gradient from buffer A to buffer B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Sample: mAb in mouse ascites (dilution, ×5)
Load volume: 100 µL

Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
PD15 TOYOPEARL GigaCap DEAE-650M Performance Comparison to TOYOPEARL DEAE-650MPerformance Data
RIS0021 TOYOPEARL DEAE-650Resin Information Sheet
TP191 Characterization of a Novel High Capacity Weak Anion Exchange Resin Technical Presentation
P/NDescriptionPrice ($) 
21360ToyoScreen DEAE-650M, 1 mL (qty. 6)167
21361ToyoScreen DEAE-650M, 5 mL (qty. 6)350
21392ToyoScreen IEC Anion Mix Pack, 1 mL (qty. 5)160
21393ToyoScreen IEC Anion Mix Pack, 5 mL (qty. 5)334