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TOYOPEARL GigaCap DEAE-650M

TOYOPEARL GigaCap DEAE-650M media is a high capacity anion exchange resin optimized for the purification of proteins, including monoclonal antibodies.  The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been chemically modified to provide a higher number of anionic binding sites and functionalized with diethyl amino ethyl (DEAE) weak anion exchange groups.

TOYOPEARL GigaCap DEAE-650 resin can be used for high throughput capture and intermediate purification of blood products and other proteins. TOYOPEARL GigaCap DEAE-650 resin is available in one particle size, 75 µm, which corresponds to the M-grade designation.

TOYOPEARL GigaCap DEAE-650M exhibits typical dynamic binding capacities > 156 g/L for BSA, maintains high capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.  

TOYOPEARL GigaCap DEAE-650M also has excellent binding and elution kinetics, which results in a more concentrated protein fraction collected during the elution step.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
22865TOYOPEARL GigaCap DEAE-650M100 mL75  461
22866TOYOPEARL GigaCap DEAE-650M250 mL75  858
22867TOYOPEARL GigaCap DEAE-650M1 L75  -
22868TOYOPEARL GigaCap DEAE-650M5 L75  -
22869TOYOPEARL GigaCap DEAE-650M50 L75  -
               

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 75 µm
Ligand type: weak anion
Ligand pKa: 11.5
DBC: > 156 g/L
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life (estimated): 10 years

Structure:

IEX_GigaCap-DEAE-650M_Structure.png

 
 
 

           

Selectivity Comparison

IEX_GigaCap-DEAE-650M-Fig20.png

Resins: TOYOPEARL GigaCap DEAE-650M
TOYOPEARL GigaCap Q-650M

Capto DEAE
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 50 mmol/L Tris-HCl buffer, pH 8.5
Buffer B: buffer A + 1.0 mol/L NaCl, pH 8.5
Gradient: 120 minutes, 0 - 100% B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Injection vol.: 100 µL
Samples: 1. transferrin, 2.9 g/L
2. ovalbumin, 6.5 g/L
3. trypsin inhibitor, 10.0 g/L
Sample load: 1.94 mg total protein



Pressure-Flow Curves

IEX_GigaCap-DEAE-650M-Fig22.png

Resin: TOYOPEARL GigaCap DEAE-650M
Column size: 22 mm ID × 20 cm
Mobile phase: 0.1 mol/L NaCl
Detection: pressure (MPa)

DBC vs. Flow Rate and Residence Time

IEX_GigaCap_DEAE-650M_Fig21.png

Resin: TOYOPEARL GigaCap DEAE-650M
Column size: 6.0 mm ID × 4 cm
Mobile phase: Buffer A: 0.05 mol/L Tris-HCl buffer,
pH 8.5
Buffer B: mobile phase A +
1.0 mol/L NaCl
Flow rates: 212, 407, 623, and 739 cm/hr (1.0, 1.9, 2.9, 3.5 mL/min)
Detection: UV @ 280 nm
Sample: BSA (1.0 g/L)

Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
PD14 TOYOPEARL GigaCap DEAE-650M Preserving Resolution at Increased Protein LoadsPerformance Data
PD15 TOYOPEARL GigaCap DEAE-650M Performance Comparison to TOYOPEARL DEAE-650MPerformance Data
PO34 TOYOPEARL GigaCap DEAE-650MProduct Overview
RIS0035 TOYOPEARL GigaCap-DEAE-650MResin Information Sheet
TP191 Characterization of a Novel High Capacity Weak Anion Exchange Resin Technical Presentation
P/NDescriptionPrice ($) 
OC41MDGCDE-650MResin Seeker TOYOPEARL GigaCap DEAE-650M 96-well plate kit286
45226SkillPak AEX 1 mL column library305
45210SkillPak TOYOPEARL GigaCap DEAE-650M 1 mL columns (qty. 5)253
45246SkillPak TOYOPEARL GigaCap DEAE-650M 5 mL column210
22872ToyoScreen GigaCap DEAE-650M, 1 mL (qty. 6)161
22873ToyoScreen GigaCap DEAE-650M, 5 mL (qty. 6)338