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TSKgel SuperQ-5PW (30)

The TSKgel SuperQ-5PW (30) resin is a strong anion exchange resin for biomolecule purification. This resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with quaternary amine (Q) strong anion exchange groups.  This resin has a 100 nm pore size and a 30 µm bead size.

TSKgel SuperQ-5PW (30) resin is best used for high resolution process polishing steps and the capture of oligonucleotides from crude harvest. If a product with less resolution is needed, the larger particle sized TOYOPEARL SuperQ-650 resins may be used.  

TSKgel SuperQ-5PW (30)resin has high chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates.  TSKgel PW resins vary from TOYOPEARL resins by having a higher degree of crosslinking which is necessitated by the higher flow pressures needed when using smaller particles for chromatography. The increased level of crosslinking decreases the number of sites available for ligand immobilization when producing TSKgel PW ion exchange chromatography resins.

A TSKgel PW resin will have a lower dynamic binding capacity than the corresponding TOYOPEARL material, however; chromatographic efficiency, peak width, and resolution are improved because of their smaller particle sizes. In this way they are higher performing chromatography resins than their TOYOPEARL counterparts.

Because similarly functionalized TSKgel PW and TOYOPEARL resin types have the same backbone polymer chemistry, the selectivity for proteins and impurities remains the same.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
43283TSKgel SuperQ-5PW (30)25 mL30  248
18536TSKgel SuperQ-5PW (30)250 mL30  1,463
18548TSKgel SuperQ-5PW (30)1 L30  -
18549TSKgel SuperQ-5PW (30)5 L30  -
                   

Product Attributes

Base bead: 5000PW
Pore size (mean): 100 nm
Particle size (mean): 30 µm
Ligand type: strong anion
Ligand pKa: 12.2
DBC: 52 - 88 g/L
Pressure rating: 2.0 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years

Structure:

IEX_SuperQ-5PW20-30_Structure.png

 
 
 
 

    

Scale Up or Down using the Same Ligand


IEX_SuperQ-5PW-Fig1.png

Resins: A. TOYOPEARL SuperQ-650C, 100 µm
B. TOYOPEARL SuperQ-650M, 65 µm
C. TOYOPEARL SuperQ-650S, 35 µm
D. TSKgel SuperQ-5PW (30), 30 µm
E. TSKgel SuperQ-5PW (20), 20 µm
F. TSKgel SuperQ-5PW, 10 µm
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 0.02 mol/L Tris-HCl, pH 8.5
Buffer B: 0.5 mol/L NaCl in buffer A
Gradient: 60 min linear gradient from buffer A to buffer B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Sample: mAb in mouse ascites (dilution, × 5)
Load volume: 100 µL 


Code #DescriptionLiterature Type
AN024 Preserving Chromatographic Selectivity While Improving Your Process PurificationApplication Note
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
RIS0032 TSKgel SuperQ-5PWResin Information Sheet
TP121 High Resolution Anion Exchange Chromatography Purification of OligonucleotidesTechnical Presentation
P/NDescriptionPrice ($) 
43280TSKgel IEXPAK PW, 30 µm 634