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TSKgel DEAE-5PW (20)

TSKgel DEAE-5PW (20) resin is a weak anion exchange resin for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with diethyl amino ethyl (DEAE) weak anion exchange groups.  This resin has a 100 nm pore size and a 20 µm bead size.

TSKgel DEAE-5PW (20) resin has a high chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates.  TSKgel PW resins vary from TOYOPEARL resins by having a higher degree of crosslinking which is necessitated by the higher flow pressures needed when using smaller particles for chromatography. The increased level of crosslinking decreases the number of sites available for ligand immobilization when producing TSKgel PW ion exchange chromatography resins.

A TSKgel PW resin will have a lower dynamic binding capacity than the corresponding TOYOPEARL material, however; chromatographic efficiency, peak width, and resolution are improved because of their smaller particle sizes. In this way, they are higher performing chromatography resins than their TOYOPEARL counterparts.

TSKgel DEAE-5PW (20) resin is best used for high resolution process polishing steps. If a product with less resolution is needed, the larger particle TSKgel SP-5PW (30), TOYOPEARL DEAE-650, or TOYOPEARL GigaCap DEAE resins may be used.

Because similarly functionalized TSKgel PW and TOYOPEARL resin types have the same backbone polymer chemistry, the selectivity for proteins and impurities remains the same.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
43381TSKgel DEAE-5PW (20)25 mL20  368
14710TSKgel DEAE-5PW (20)250 mL20  2,694
14711TSKgel DEAE-5PW (20)1 L20  -
18436TSKgel DEAE-5PW (20)5 L20  -
                     

Product Attributes

Base bead: 5000PW
Pore size (mean): 100 nm
Particle size (mean): 20 µm
Ligand type: weak anion
Ligand pKa: 11.5
DBC: 25 - 45 g/L
Pressure rating: 2.0 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years

Structure:

IEX_DEAE-5PW20-30_Structure.png

 
 
 
 

    

Scale Up or Down using the Same Ligand


IEX_DEAE-5PW_selectivity.png

Resins: TOYOPEARL DEAE-650C, 100µm
TOYOPEARL DEAE-650M, 65 µm
TOYOPEARL DEAE-650S, 35 µm
TSKgel DEAE-5PW (30), 30µm
TSKgel DEAE-5PW (20), 20µm
TSKgel DEAE-5PW, 10µm
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 0.02 mol/L Tris-HCl, pH 8.5
Buffer B: 0.5 mol/L NaCl in buffer A
Gradient: 60 min linear gradient from buffer A to buffer B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Sample: mAb in mouse ascites (dilution, ×5)
Load volume: 100 µL

Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
RIS0030 TSKgel DEAE-5PWResin Information Sheet
P/NDescriptionPrice ($) 
43380TSKgel IEXPAK PW, 20 µm 940