Process Media


TOYOPEARL GigaCap S-650S resin is a high capacity, high resolution cation exchange resin optimized for the purification of both small and large proteins, including monoclonal antibodies.  TOYOPEARL GigaCap S-650S resin is composed of a base material of hydroxylated methacrylic polymer beads that have been chemically modified to provide a higher number of cationic binding sites and functionalized with sulfopropyl (S) strong cation exchange groups.  TOYOPEARL GigaCap-650S has a 100 nm pore size and a 35 µm particle size and can be used for high resolution intermediate purification and polishing process steps.

TOYOPEARL GigaCap S-650S has a dynamic binding capacity greater than 160 g/L for human IgG , maintains high capacity at increased linear velocities, has good pressure-flow characteristics and will withstand back pressures up to 0.3 MPa. 

This high capacity, combined with the 35 µm mean particle size, makes TOYOPEARL GigaCap S-650S ideal for high resolution applications.

TOYOPEARL GigaCap S-650S also has excellent binding and elution kinetics, which results in a more concentrated protein fraction collected during the elution step.


P/NDescriptionResin VolumeParticle Size (µm)   Price
0022875TOYOPEARL GigaCap S-650S25 mL35  261
0022876TOYOPEARL GigaCap S-650S250 mL35  1,933
0022877TOYOPEARL GigaCap S-650S1 L35  -
0022878TOYOPEARL GigaCap S-650S5 L35  -
0022879TOYOPEARL GigaCap S-650S50 L35  -


Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 35 µm
Ligand type: strong cation
Ligand pKa: 1.2
DBC: > 150 g/L
Shipping buffer: 20% ethanol
pH stability: 3-13
Stability data (estimated): 10 years





Dynamic Binding Capacity at Various Linear Velocities


Resin: TOYOPEARL GigaCap S-650S
Column size: 6.0 mm ID × 4 cm
Mobile phase: A: 50 mmol/L acetate buffer, pH 4.7
B: mobile phase A + 0.5 mol/L NaCl
Flow rates: 211, 425, 637 cm/hr 
Residence times: 1.1 min, 0.6 min, and 0.4 min
Detection: UV @ 280 nm
Sample: γ globulin, 1.0 g/L

Pressure-Flow Comparison


Resins: TOYOPEARL GigaCap S-650S
SP Sepharose HP
Column size: 22 mm ID × 20 cm
Mobile phase: 0.1 mol/L NaCl
Detection: pressure (MPa)

Code #DescriptionLiterature Type
AN069 TOYOPEARL GigaCap Cation Exchange Resins for Aggregate Removal from Monoclonal Antibodies Application Note
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
PO37 TOYOPEARL GigaCap S-650SProduct Overview
RIS0024 TOYOPEARL GigaCap-S-650Resin Information Sheet
TP192 Characterization of Two Novel High Capacity, High Resolution Strong Ion Exchange Resins Technical Presentation
P/NDescriptionPrice ($) 
0045228SkillPak Antibody 1 mL column library434
0045227SkillPak CEX 1 mL column library341
0045204SkillPak TOYOPEARL GigaCap S-650S 1 mL columns (qty. 5)284
0045240SkillPak TOYOPEARL GigaCap S-650S 5 mL column235
0045023ToyoScreen RoboColumns GigaCap S-650S, 200 µL-
0045024ToyoScreen RoboColumns GigaCap S-650S, 600 µL-