Process Media


TOYOPEARL SP-550C media is a strong cation exchanger for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with sulfopropyl (S) strong cation exchange groups.  TOYOPEARL SP-550C resins have high chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates.

TOYOPEARL SP-550C is currently available in only one particle size, 100 µm, and corresponds to the C -grade assigned to resins with this particle size. TOYOPEARL SP-550C is designed to be used for the high throughput capture of product from biological feedstock.

TOYOPEARL SP-550C exhibits typical dynamic binding capacities for lysozyme of 100 g/L.  TOYOPEARL SP-550C maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.  


P/NDescriptionResin VolumeParticle Size (µm)   Price
0043272TOYOPEARL SP-550C100 mL100  242
0014028TOYOPEARL SP-550C250 mL100  545
0014705TOYOPEARL SP-550C1 L100  -
0014029TOYOPEARL SP-550C5 L100  -
0018366TOYOPEARL SP-550C50 L100  -

Product Attributes

Base bead: HW-55
Pore size (mean): 50 nm
Particle size (mean): 100 µm
Ligand type: strong cation
Ligand pKa: 1.2
DBC: 80 - 120 g/L
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years





Dynamic Binding Capacity Comparison

Influence of pore size on lysozyme DBC 


Mobile phase: 50 mmol/L phosphate buffer, pH 6.0
Flow rate: 100 cm/hr
Sample: lysozyme, 1 mg/mL

Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
RIS0027 TOYOPEARL SP-550CResin Information Sheet
P/NDescriptionPrice ($) 
OC41MDCEX-96Resin Seeker TOYOPEARL CIEX 96-well plate kit426
0045213SkillPak TOYOPEARL SP-550C 1 mL columns (qty. 5)270
0045249SkillPak TOYOPEARL SP-550C 5 mL column223
0021394ToyoScreen IEC Cation Mix Pack, 1 mL (qty. 5)-
0021395ToyoScreen IEC Cation Mix Pack, 5 mL (qty. 5)-
0021396ToyoScreen IEC Mix Pack, 1 mL (qty. 5)-
0021397ToyoScreen IEC Mix Pack, 5 mL (qty. 5)-
0021370ToyoScreen SP-550C, 1 mL (qty. 6)-
0021371ToyoScreen SP-550C, 5 mL (qty. 6)-