Process Media


TOYOPEARL CM-650M resin is a weak cation exchanger for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with carboxymethyl (CM) weak cation exchange groups.  TOYOPEARL CM-650M has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates.

TOYOPEARL CM-650M has a 100 nm pore size and a 65 µm bead size and can be used for high throughput intermediate purification, and polishing process steps. If greater resolution is needed, the smaller particle TOYOPEARL CM-650S resin may be used.

TOYOPEARL CM-650M has a dynamic binding capacity for lysozyme of 40 g/L.  TOYOPEARL CM-650M maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.  


P/NDescriptionResin VolumeParticle Size (µm)   Price
0043203TOYOPEARL CM-650M100 mL65  215
0007475TOYOPEARL CM-650M250 mL65  490
0014696TOYOPEARL CM-650M1 L65  -
0007972TOYOPEARL CM-650M5 L65  -
0019839TOYOPEARL CM-650M50 L65  -

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 65 µm
Ligand type: weak cation
Ligand pKa: 4.7
DBC: 30 - 50 g/L
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13





Dynamic Binding Capacity Comparison

Influence of pore size on lysozyme DBC 


Mobile phase: 50 mmol/L phosphate buffer, pH 6.0
Flow rate: 100 cm/hr
Sample: lysozyme, 1 mg/mL

Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
RIS0020 TOYOPEARL CM-650Resin Information Sheet
P/NDescriptionPrice ($) 
0021366ToyoScreen CM-650M, 1 mL (qty. 6)-
0021367ToyoScreen CM-650M, 5 mL (qty. 6)-
0021394ToyoScreen IEC Cation Mix Pack, 1 mL (qty. 5)-
0021395ToyoScreen IEC Cation Mix Pack, 5 mL (qty. 5)-