Process Media


TOYOPEARL CM-650C resin is a weak cation exchanger for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with carboxymethyl (CM) weak cation exchange groups.  TOYOPEARL CM-650C has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates.

TOYOPEARL CM-650C has a 100 nm pore size and a 100 µm bead size and can be used for high throughput capture, intermediate purification, and polishing process steps.  If greater resolution is needed, the smaller particle TOYOPEARL CM-650M or TOYOPEARL CM-650S resins may be used.

TOYOPEARL CM-650C has a dynamic binding capacity for lysozyme of 35 g/L. TOYOPEARL CM-650C maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.  


P/NDescriptionResin VolumeParticle Size (µm)   Price
07991TOYOPEARL CM-650C250 mL100  427
14697TOYOPEARL CM-650C1 L100  -
07992TOYOPEARL CM-650C5 L100  -
19329TOYOPEARL CM-650C50 L100  -

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 100 µm
Ligand type: weak cation
Ligand pKa: 4.7
DBC: 25 - 45 g/L
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13



Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
RIS0020 TOYOPEARL CM-650Resin Information Sheet
P/NDescriptionPrice ($) 
OC41MDCEX-96Resin Seeker TOYOPEARL CIEX 96-well plate kit384