Process Media

TSKgel SP-5PW (20)

TSKgel SP-5PW (20) resin is a strong cation exchange resin for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with sulfopropyl (S) strong cation exchange groups.  This resin has a 100 nm pore size and a 20 µm bead size.

TSKgel SP-5PW (20) resin has a high chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates.  TSKgel PW resins vary from Tosoh’s TOYOPEARL resins by having a higher degree of crosslinking which is necessitated by the higher flow pressures needed when using smaller particles for chromatography. The increased level of crosslinking decreases the number of sites available for ligand immobilization when producing TSKgel PW ion exchange chromatography resins.

A TSKgel PW resin will have a lower dynamic binding capacity than the corresponding TOYOPEARL material, however; chromatographic efficiency, peak width, and resolution are improved because of their smaller particle sizes. In this way they are higher performing chromatography resins than their TOYOPEARL counterparts.

TSKgel SP-5PW (20) resin is best used for high resolution process polishing steps. If a product with less resolution is needed, the larger particle TSKgel SP-5PW (30) or TOYOPEARL SP-650 resins may be used.

Because similarly functionalized TSKgel PW and TOYOPEARL resin types have the same backbone polymer chemistry, the selectivity for proteins and impurities remains the same.


P/NDescriptionResin VolumeParticle Size (µm)   Price
43382TSKgel SP-5PW (20)25 mL20  384
14714TSKgel SP-5PW (20)250 mL20  2,826
14715TSKgel SP-5PW (20)1 L20  -
18435TSKgel SP-5PW (20)5 L20  -

Product Attributes

Base bead: 5000PW
Pore size (mean): 100 nm
Particle size (mean): 20 µm
Ligand type: strong cation
Ligand pKa: 1.2
DBC: 20 - 40 g/L
Pressure rating: 2.0 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years




Code #DescriptionLiterature Type
AN024 Preserving Chromatographic Selectivity While Improving Your Process PurificationApplication Note
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
PD05 Optimizing Pore Size to Improve Throughput in Downstream Purification of Biological Therapeutic ProductsPerformance Data
RIS0031 TSKgel SP-5PWResin Information Sheet
P/NDescriptionPrice ($) 
45207SkillPak TSKgel SP-5PW (20) 1 mL columns (qty. 5)277
45243SkillPak TSKgel SP-5PW (20) 5 mL column234
43380TSKgel IEXPAK PW, 20 µm 940