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TSKgel SP-3PW (30)

TSKgel SP-3PW (30) media is a strong cation exchange resin for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with sulfopropyl (S) strong cation exchange groups. 

TSKgel SP-3PW (30) resin has high chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates.  TSKgel PW resins vary from TOYOPEARL resins by having a higher degree of crosslinking which is necessitated by the higher flow pressures needed when using smaller particles for chromatography. The increased level of crosslinking decreases the number of sites available for ligand immobilization when producing TSKgel PW ion exchange chromatography resins.

A TSKgel PW resin will have a lower dynamic binding capacity than the corresponding TOYOPEARL material, however; chromatographic efficiency, peak width, and resolution are improved because of their smaller particle sizes. In this way they are higher performing chromatography resins than their TOYOPEARL counterparts.

TSKgel SP-3PW (30) resin is best used for high resolution process polishing steps for peptide purifications such as insulin. TSKgel SP-3PW (30) resin is available in one particle sizes, 30 µm, and has a smaller pore size (25 nm) than the TSKgel SP-5PW (100 nm).  

Because similarly functionalized TSKgel PW and TOYOPEARL resin types have the same backbone polymer chemistry, the selectivity for proteins and impurities remains the same, though the TSKgel SP-3PW (30) has no TOYOPEARL analogue. 

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
21976TSKgel SP-3PW (30)25 mL30  259
21977TSKgel SP-3PW (30)250 mL30  1,887
21978TSKgel SP-3PW (30)1 L30  -
21979TSKgel SP-3PW (30)5 L30  -
21980TSKgel SP-3PW (30)50 L30  -
            

Product Attributes

Base bead: 3000PW
Pore size (mean): 25 nm
Particle size (mean): 30 µm
Ligand type: strong cation
Ligand pKa: 1.2
DBC: > 65 g/L
Pressure rating: 2.0 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life (estimated): 10 years

Structure:

IEX_SP-3PW30_Structure.png

 
 
 

           

Insulin Purification

TSKgel SP-3PW (30) resin was developed as a higher resolving and higher capacity resin for insulin purification.

Selectivity comparison

IEX_SP-3PW-Fig34.png




Resins: A. TSKgel SP-3PW (30)
B. SOURCE 30S
C. TSKgel SP-5PW (30)
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 0.02 mol/L sodium citrate buffer, pH 3.2 + ethanol = 8/2 (v/v)
Buffer B: 0.02 mol/L sodium citrate buffer, pH 3.2 + 1.0 mol/L NaCl/ethanol = 8/2 (v/v)
Gradient: 60 min linear gradient from buffer A to buffer B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Samples: 1. trypsinogen  
2. insulin  
3. lysozyme
Sample vol.: 100 µL (0.5 g/L each)



Dynamic binding capacity comparison


Resin TSKgel SP-3PW (30) TSKgel SP-5PW (30) SOURCE 30S
Matrix polymethacrylate polymethacrylate polystyrene
divinylbenzene
Particle
size

30 µm 30 µm 30 µm
Insulin
capacity
49 g/L 24 g/L 45 g/L
Pore
size

25 nm 100 nm NR




Dynamic binding capacities were determined at 10% breakthrough.

Column size: 4.6 mm ID × 7.5 cm
Mobile phase: gradient elution with 1-propanol by acidic buffer, pH 3.0 containing neutral salt
Flow rate: 270 cm/hr (0.75 mL/min)
Sample: recombinant insulin (7.2 g/L)

For additional applications, please see our applications database.

 
Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
PO26 TSKgel SP-3PW (30) ResinProduct Overview
RIS0034 TSKgel SP-3PWResin Information Sheet