Process Media


TOYOPEARL AF-Chelate-650M resin carries a chelating ligand, iminodiacetic acid (IDA), that can form stable chelate complexes with selected metal ions such as Cu2+, Ni2+, Zn2+ and Co2+. The resultant resin can be used for immobilized metal affinity chromatography (IMAC). It binds to histidine rich/histidine tagged proteins and to cysteine containing proteins.


P/NDescriptionResin VolumeParticle Size (µm)   Price
0014475TOYOPEARL AF-Chelate-650M25 mL65  224
0019800TOYOPEARL AF-Chelate-650M100 mL65  634
0014907TOYOPEARL AF-Chelate-650M1 L65  -
0014908TOYOPEARL AF-Chelate-650M5 L65  -


Product Attributes

Pore size (mean): 100 nm
Particle size (mean): 65 µm
Shipping buffer: 20% ethanol
pH stability: 2-12
Stability data: 10 years




Separation of Two Proteins


Resin: TOYOPEARL AF-Chelate-650M
Column size: 8 mm ID × 7.5 cm
Metal ion: Zn+2
Mobile phase: Buffer A: 20 mmol/L Tris-HCl, 0.5 mol/L NaCl, pH 8.0
Buffer B: buffer A + 0.2 mol/L glycine
Gradient: 0 - 100% B (120 min)
Flow rate: 60 cm/hr (0.5 mL/min)
Detection: UV @ 280 nm
Sample: 1. ribonuclease A, 250 µg
2. transferrin, 250 µg

For additional applications, please see our applications database.

Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM08 Instruction Manual -- TOYOPEARL AF-Chelate-650MInstruction Manual
PCAT04 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- AffinityProduct Catalog
RIS0003 TOYOPEARL AF-Chelate-650MResin Information Sheet
P/NDescriptionPrice ($) 
0045202SkillPak TOYOPEARL Chelate 650M 1 mL column133
0045259SkillPak TOYOPEARL Chelate 650M 5 mL column384
0021384ToyoScreen AF-Chelate-650M, 1 mL (qty. 6)-
0021385ToyoScreen AF-Chelate-650M, 5 mL (qty. 6)-