Purification of Lectins
|
| Resin: |
GaINAc-Epoxy TOYOPEARL |
| Column size: |
10 mm ID × 5 cm |
| Mobile phase: |
Buffer A: 10 mmol/L phosphate-buffered saline, pH 7.4
Buffer B: buffer A + 0.1 mol/L lactose |
| Gradient: |
equilibrate/load/wash 100% buffer A
isocratic elution 100% buffer B |
| Detection: |
UV @ 275 nm |
| Sample: |
4.0 mg impure Grifola frondosa lectin |
|
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