Purification of Ferredoxin-NADP Reductase
|
|
| Resin: |
Synechococcus Fd-TOYOPEARL |
| Column size: |
22 mm ID × 10 cm |
| Mobile phase: |
1. Load with crude spinach FNR in
20 mmol/L Tris-HCl, pH 7.5
2. Wash with 20 mmol/L Tris-HCl,
pH 7.5
3. Elute with 20 mmol/L Tris-HCl, pH 7.5 with 0.25 mol/L NaCl |
| Flow rate: |
16 cm/hr (1 mL/min) |
| Detection: |
UV @ 275 nm, specific activity |
| Sample: |
spinach FNR |
|
For additional applications, please see our applications database.