Purification of Ferredoxin-NADP Reductase
![AFC_AF-Tresyl-650_Fig12_rev.png](https://separations.us.tosohbioscience.com/Image Library/TBL/Product Catalog/Process Media/Affinity/AFC_AF-Tresyl-650_Fig12_rev.png)
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Resin: |
Synechococcus Fd-TOYOPEARL |
Column size: |
22 mm ID × 10 cm |
Mobile phase: |
1. Load with crude spinach FNR in
20 mmol/L Tris-HCl, pH 7.5
2. Wash with 20 mmol/L Tris-HCl,
pH 7.5
3. Elute with 20 mmol/L Tris-HCl, pH 7.5 with 0.25 mol/L NaCl |
Flow rate: |
16 cm/hr (1 mL/min) |
Detection: |
UV @ 275 nm, specific activity |
Sample: |
spinach FNR |
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