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TSKgel Phenyl-3PW (20)

TSKgel® Phenyl-3PW (20) resin is a hydrophobic interaction chromatography (HIC) resin designed for oligonucleotide purification. This resin features phenyl group ligands functionalized on a 20 µm polymethacrylate base particle with 25 nm pores, which binds approximately 2.5 times more oligonucleotides than TSKgel Phenyl-5PW.

TSKgel Phenyl-3PW (20) resin efficiently purifies oligonucleotides with both DMT and MMT protection groups, ensuring high purity and yield. Its versatility reduces the need for multiple resins, streamlining processes for manufacturers and improving process economics.

TSKgel PW resins’ high degree of crosslinking results in high chemical and mechanical stability, allowing rigorous cleaning-in-place (CIP) and fast flow rates.
 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)		 
0023566TSKgel Phenyl-3PW (20)25 mL20  361
0023568TSKgel Phenyl-3PW (20)250 mL20  2,514
0023569TSKgel Phenyl-3PW (20)1 L20  -
0023570TSKgel Phenyl-3PW (20)5 L20  -
0023571TSKgel Phenyl-3PW (20)25 L20  -
0023572TSKgel Phenyl-3PW (20)50 L20  -

           

Product Attributes

Base bead: 3000PW
Pore size (mean): 25 nm
Particle size (mean): 20 µm 
Ligand type: Phenyl
DBC: 25-45 g/L 
Shipping buffer: 20% ethanol
pH stability: 1-13

Structure:

HIC_Phenyl-3PW_Structure.png

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

 
 
 

    

On-column Purification and DMT Cleavage (10 mg-oligo/mL-resin load)

HIC_Phenyl-3PW_App1-rev.png
Resin: TSKgel Phenyl-3PW (20)
Column size: 5 mm ID × 5 cm (1 mL)
Flow rate: 1 mL/min (300 cm/hr)
Detection: UV @ 254 nm (mAU), pH
Temperature: ambient
Instrument: ÄKTA avant 25 (Unicorn 7.3)

Phase Mobile phase CV
Equilibration 10 mmol/L NaOH, 1.0 mol/L (NH4)2SO4 10
Load DMT-protected 20-mer,
20 mL @ 0.5 mg/mL (10 mg-oligo / mL-resin)		 2
Wash 10 mmol/L NaOH, 1.0 mol/L (NH4)2SO4 5
Cleavage 200 mmol/L acetic acid, 1.0 mol/L (NH4)2SO4 10*
CIP1 water 5
CIP2 0.5 mol/L arginine 5

*A pH 4.0 hold for 120 min was included after 2 CVs.
 
Code #DescriptionLiterature Type
RIS0049 TSKgel Phenyl-3PW (20)Resin Information Sheet
TP233 One-step DMT-protected oligonucleotide purification and simultaneous on-column detritylation using hydrophobic interaction chromatographyTechnical Presentation
 

Tosoh Bioscience LLC
 3604 Horizon Drive, Suite 100
King of Prussia, PA 19406

Phone: 800-366-4875

Email: [email protected]