Products

Process Media

TOYOPEARL Super A

TOYOPEARL® Super A resin provides a cutting-edge experience in protein A media, blending top-tier critical performance attributes with exceptional operational flexibility.

An enhanced, animal-origin free, recombinant protein A ligand is bound to the polymethacrylate base bead via multipoint attachment resulting in excellent base stability for up to 100 CIP cycles with 0.5 mol/L NaOH and up to 70 CIP cycles with 1 mol/L NaOH while maintaining 90 % of its initial DBC.

TOYOPEARL Super A resin is available in ~45 µm particle size (F-grade) and exhibits dynamic binding capacities of greater than 70 g/L at 5 minutes residence time and greater than 60 g/L at 3 minutes residence time. Improved mass transfer characteristics allow it to maintain a larger percent of its capacity at lower residence times relative to comparable agarose-based resins. It is therefore ideally suited for continuous operation using multiple short columns. This resin is also available in pre-packed SkillPak columns to simplify your process development and scale-up.

Highlights

  • State-of-the-art operational flexibility: Supports high titers with high dynamic binding capacity (DBC), enhanced alkaline stability for rigorous CIP, and excellent pressure flow behavior to increase process efficiency.
  • Superior critical process performance: Features mild elution conditions, concentrated elution volumes, and a significant reduction in process impurities, making the process robust, safe, and compliant.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
0023580TOYOPEARL Super A10 mL45  762
0023581TOYOPEARL Super A25 mL45  1,313
0023582TOYOPEARL Super A100 mL45  2,626
0023583TOYOPEARL Super A1 L45  -
0023584TOYOPEARL Super A5 L45  -


Product Attributes

Matrix: hydroxylated methacrylic polymer
Ligand: modified, code-optimized C domain, hexameric ligand with multipoint attachment
Average particle size: ~45 µm
Average pore size: 100 nm
Dynamic binding capacity: 72 mg mAb/mL resin @ 5 min RT
60 mg mAb/mL resin @ 3 min RT
Operating linear flow rate: 10 – 600 cm/hr
pH stability (operational): 3-12
pH stability (CIP): 2-14
Chemical stability: stable to all commonly used aqueous buffers in protein A chromatography
Bacterial count: max 100 CFU/mL
Endotoxin concentration: max 10 EU/mL
Delivery conditions: 72% slurry in 20% ethanol

      

Dynamic Binding Capacity

DBC-TOYOPEARL-Super-A.png

Resin: TOYOPEARL Super A
Column size: 0.6 cm ID × 4.0 cm L

 

Alkaline Stability

Alkaline-Stability-TOYOPEARL-Super-A.png

Relative DBC in dependence of the number of CIP cycles performed with 0.5 M and 1M NaOH at contact time 15 min for each cycle.

Code #DescriptionLiterature Type
PD23 TOYOPEARL Super A Resin: Unmatched Performance in Protein A ChromatographyPerformance Data
PO54 TOYOPEARL Super A Resin: An Efficient and Durable Protein A ResinProduct Overview
RIS0048 TOYOPEARL Super AResin Information Sheet