Applications Database

G00006

 

Data code:

Separation of analgesics (oxyphenbutazone, phenylbutazone) by reversed-phase chromatography

1) Oxyphenbutazone; 2) Phenylbutazone

 
Chromatographic conditions
Column:TSKgel ODS-100S, P/N 19534, 5 µm, 4.6 mm ID × 15 cm × 1
Mobile Phase:Methanol / 50 mmol/L sodium dihydrogen phosphate (pH 2.5 with phosphoric acid) = 70 / 30
Flow Rate:1.0 mL/min
Detection: UV/VIS @ 254 nm
Temperature:40 °C
Injection Volume:10 µL
Sample(s):Standard
Sample Load:0.05 g/L