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G00008

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Data code:

Separation of 8-chlortheophylline and phenobarbital by reversed-phase chromatography

1) 8-Chlortheophylline; 2) Phenobarbital

 
Chromatographic conditions
Column:TSKgel ODS-100S, P/N 19534, 5 µm, 4.6 mm ID × 15 cm × 1
Mobile Phase:Methanol / 50 mmol/L sodium dihydrogen phosphate (pH 2.5 with phosphoric acid) = 50 / 50
Flow Rate:1.0 mL/min
Detection: UV/VIS @ 254 nm
Temperature:40 °C
Injection Volume:10 µL
Sample(s):Standard
Sample Load:1) 0.01 g/L, 2) 0.05 g/L