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G00010

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Data code:

Separation of acetamidophenols by revered-phase chromatography

1) p-Acetamidophenol; 2) m-Acetamidophenol; 3) o-Acetamidophenol

 
Chromatographic conditions
Column:TSKgel ODS-100S, P/N 19534, 5 µm, 4.6 mm ID × 15 cm × 1
Mobile Phase:Methanol / 50 mmol/L sodium dihydrogen phosphate (pH 2.5 with phosphoric acid) = 30 / 70
Flow Rate:1.0 mL/min
Detection: UV/VIS @ 254 nm
Temperature:40 °C
Injection Volume:10 µL
Sample(s):Standard
Sample Load:1) 0.01 g/L, 2) 0.02 g/L, 3) 0.05 g/L