Applications Database

G00037

 

Data code:

Separation of antipyretic analgesics (p-Acetaminophen, Caffeine, o-Ethoxy benzamide) by reversed-phase chromatography

1) p-Acetaminophen; 2) Caffeine; 3) o-Ethoxy benzamide

 
Chromatographic conditions
Column:TSKgel ODS-80Ts, P/N 18150, 5 µm, 2.0 mm ID × 15 cm × 1
Mobile Phase:Acetonitrile / 50 mmol/L sodium dihydrogen phosphate (pH 2.5 with phosphoric acid) = 20 / 80
Flow Rate:0.19 mL/min
Detection: UV/VIS @ 254 nm
Temperature:40 °C
Injection Volume:2 µL
Sample(s):Standard
Sample Load:1) 0.005 g/L, 2) 0.02 g/L, 3) 0.1 g/L
Comments:UV: micro flow cell