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G00063

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1
 

Data code:

Separation of theobromine, theophylline, caffeine by reversed-phase chromatography

1) Theobromine; 2) Theophylline; 3) Caffeine

 
Chromatographic conditions
Column:TSKgel Super-ODS, P/N 18197, 2.3 µm, 4.6 mm ID × 10 cm × 1
Mobile Phase:Acetonitrile / 0.1 mol/L potassium dihydrogen phosphate (pH 5.3 with potassium hydroxide) = 10 / 90
Flow Rate:1.0 mL/min
Detection: UV/VIS @ 273 nm
Temperature:40 °C
Injection Volume:7 µL
Sample(s):Standard
Sample Load:0.1 g/L
Comments:UV: micro flow cell