| https://separations.us.tosohbioscience.com

Data code:

G00068

Application Title:

Separation of water soluble vitamins by reversed-phase chromatography

Compound:

1) Vitamin C; 2) Vitamin B1; 3) Vitamin B6; 4) Vitamin B12; 5) Vitamin B2

Chromatographic conditions
Column:	TSKgel ODS-100S, P/N 19534, 5 µm, 4.6 mm ID × 15 cm × 1
Mobile Phase:	A: Acetonitrile / 0.05 mol/L sodium dihydrogen phosphate(pH 2.5 with phosphoric acid) + 0.1 mol/L sodium perchlorate = 2 / 98 B: Acetonitrile / 0.05 mol/L sodium dihydrogen phosphate (pH 2.5 with phosphoric acid) = 50 / 50 Gradient: : 0 - 5 min, A hold, 5 - 10 min, A to B linear gradient
Flow Rate:	1.0 mL/min
Detection:	UV/VIS @ 254 nm
Temperature:	40 °C
Injection Volume:	20 µL
Sample(s):	Standard
Sample Load:	1) 0.005 g/L, 2) 0.01 g/L, 3) 0.1 g/L