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G00090

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Data code:

Separation of antidepressant by reversed-phase chromatography

1) Doxepin; 2) Imipramine; 3) Desipramine; 4) Trimipramine; 5) Nortriptyline

 
Chromatographic conditions
Column:TSKgel ODS-100S, P/N 19534, 5 µm, 4.6 mm ID × 15 cm × 1
Column 2:TSKgel ODS-100S, P/N 19536, 5 µm, 3.0 mm ID × 15 cm × 1
Column 3:TSKgel ODS-100S, P/N 19538, 5 µm, 2.0 mm ID × 15 cm × 1
Mobile Phase:50 mmol/L Sodium Dihydrogenphosphate(pH 2.2) / Methanol = 50 / 50
Flow Rate:1) 1.0 mL/min, 2) 0.425 mL/min, 3) 0.2 mL/min
Detection: UV/VIS @ 260 nm
Temperature:40 °C
Injection Volume:1) 10 µL, 2) 4 µL, 3) 2 µL
Sample(s):Standard