Column: | TSKgel ODS-80Ts QA, P/N 18520, 5 µm, 4.6 mm ID × 15 cm × 1 |
Column 2: | TSKgel ODS-80Ts QA, P/N 18768, 5 µm, 2.0 mm ID × 15 cm × 1 |
Mobile Phase: | 50 mmol/L Sodium Dihydrogenphosphate (pH 2.2) / Methanol = 80 / 20 |
Flow Rate: | 1) 1.0 mL/min, 2) 0.2 mL/min |
Detection: | UV/VIS @ 260 nm |
Temperature: | 40 °C |
Injection Volume: | 1) 10 µL, 2) 2 µL |
Sample(s): | Standard |