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Inhibitory Effect of 0.19 alfa-Amylase Inhibitor from Wheat Kernel on the Activity of Porcine Pancreas alfa-Amylase and Its Thermal Stability

0.19 alfa-Amylase Inhibitor

 
Chromatographic conditions
Column:TSKgel SuperQ-5PW, P/N 18257, 10 µm, 7.5 mm ID × 7.5 cm × 1
Column 2:TSKgel BioAssist Q, 10 mm ID × 5 cm × 1
Column 3:TSKgel G2000SWXL, P/N 08540, 7.8 mm ID × 30 cm × 1
Mobile Phase:0 mol/L to 50 mmol/L NaCl at time 5 min over 25 min
  15 mmol/L Tris-HCl buffer (pH 8.3) (buffer A)
  15 mmol/L borate buffer (pH 10.0)
  20 mmol/L sodium phosphate buffer (pH 6.9) containing 0.3 mol/L NaCl
Flow Rate:1.0 mL/min, 0.8 mL/min, 1.0 mL/min
Temperature:25 °C, 25 °C, 30 °C
Sample(s):Wheat Kernel
Reference:J.Biochem
Title:Inhibitory Effect of 0.19 alfa-Amylase Inhibitor from Wheat Kernel on the Activity of Porcine Pancreas alfa-Amylase and Its Thermal Stability
Year:2004
Volume:135
Number:3
Page start:421
Page end:427
Author:Hiroshi Oneda, Seungjae Lee, and Kuniyo Inouye.