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JB0290

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Data code:

Hydrophilic interaction liquid chromatography-mass spectrometry as a new tool for the characterization of intact semi-synthetic glycoproteins

glycoconjugate

 
Chromatographic conditions
Column:TSKgel Amide-80 3µｍ 2.0 ｘ 150, P/N 21865, 2.0ｍｍ　x　15cm
Mobile Phase:A:acetonitrile（ACN) containing 0.05％ TFA
B:water containing 0.05％ TFA
32％-42％B in 20min followed by isocratic elution at 42％B
Flow Rate:0.3mL/min
Detection: MS
Temperature:50℃
Injection Volume:2µL
Sample(s):RNase A and RNase B
Reference:Anal. Chim. Acta
Title:Hydrophilic interaction liquid chromatography-mass spectrometry as a new tool for the characterization of intact semi-synthetic glycoproteins
Year:2017
Volume:981
Page start:94
Page end:105
Author:Sara Tengattini; Elena Dominguez-Vega; Caterina Temporini; Teodara Bravaro; Francesca Rinaldi; Luciano Piubelli; Loredano Pollegioni; Gabriella Massolini; Govert W. Somsen