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T00842

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Data code:

Separation of Aldehydes and Ketones as it's DNPH or CNET derivative by HPLC

DNPH-Formaldehyde; DNPH-Acetaldehyde; DNPH-Acetone;
 DNPH-Acrolein; DNPH-Propionaldehyde; DNPH-Crotonaldehyde;
DNPH-2-Butanone; DNPH-Methacrolein; DNPH-Butylaldehyde;
 DNPH-Benzaldehyde; DNPH-Cyclohexanone; DNPH-Valeraldehyde; DNPH-p-Tolualdehyde; DNPH-Hexaldehyde

 
Chromatographic conditions
Column:TSKgel ODS-100Z 3μm (3.0 mmI.D. x 15 cm), P/N 22739, 3.0mm×15cm
Mobile Phase:

A; water/tetrahydrofuran = 80/20、B; acetonitrile、

Gradient: 0 min(B 25 %)→15 min(B 50 %)→20 min(B 70 %)→25 min(B 70 %)

Flow Rate:0.7mL/min
Detection: UV/VIS　360nm
Temperature:40°Ｃ
Injection Volume:5µL
Reference:Tosoh　Technical　Information
Title:Separation of Aldehydes and Ketones as it's DNPH or CNET derivative by HPLC
Number:156