Applications Database

T00844

 

Data code:

Simultaneous and Direct Analysis of Biogenic Amines in Food by LC-MS/MS using Hydrophilic Chromatography

Trp; Put; Cad; His; Tyr; Spd; 1,6-diaminohexane

 
Chromatographic conditions
Column:TSKgel ODS-100Z 3μm (4.6mmI.D. x 15cm), P/N 22746, 4.6mm ×15cm
Mobile Phase:

A;30mM ammonium formate in water (pH4.0)、B;acetonitrile、

Gradient ; 0 min (B 90%)→12 min(B 40%)→14 min(B 40%) →16 min(B 90%)

Flow Rate:0.2mL/min
Detection: MS
Temperature:50°C
Injection Volume:2µL
Reference:Tosoh Technical Information
Title:Simultaneous and Direct Analysis of Biogenic Amines in Food by LC-MS/MS using Hydrophilic Chromatography
Number:157