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Analysis of Phytic acid in Extraction from Rice bran by HILIC

phytic acid

 
Chromatographic conditions
Column:TSKgel Amide-80 3μm (4.6 mmI.D. x 150 mm), P/N 21867, 4.6mm ×15cm
Mobile Phase:

1 % TFA + 20 mmol/L TFA・Na in (H2O/CH3CN = 40/60)

 

reaction（2.5 mmol/L Fe(NO3)3 + 0.3 mol/L HClO4）

Flow Rate:1.0mL/min
Detection: UV/VIS　295nm
Temperature:30°C
Injection Volume:5µL
Reference:Tosoh　Technical　Information
Title:Analysis of Phytic acid in Extraction from Rice bran by HILIC
Number:165