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Data code:

DAR Analysis of Antibody-Drug Conjugate by HIC

Trastuzumab;Trastuzumab-vcMMAE;ADC

 
Chromatographic conditions
Column:TSKgel Butyl-NPR, P/N 42168, 4.6 mm I.D × 10 cm
Mobile Phase:

A) 25 mmol/L phosphate buffer (pH 7.0) including 1.5 mol/L ammonium sulfate

B) 25 mmol/L phosphate buffer (pH 7.0) / 2-propanol = 80 / 20

Gradient: 0 → 100 % B (20 minutes)

Flow Rate:0.5mL/min
Detection: UV/VIS 280nm
Injection Volume:10µL
Reference:Tosoh Technical Information
Title:DAR Analysis of Antibody-Drug Conjugate by HIC
Number:173