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T00903

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Data code:

Analysis of Catechins ｂｙ HPLC

(-)-epigallocatechin; (-)-catechin; (-)-epigallocatechin gallate; caffeine; (+)-epicatechin; (-)-epicatechin gallate; (-)-catechin gallate

 
Chromatographic conditions
Column:TSKgelODS-100Z 5μm 4.6×15, P/N 21461, 4.6mm ×15cm
Mobile Phase:A;  10mM KH2PO4(pH2.5)  B;  CH3OH Gradient: 0min(B ; 18%)→15min(B ; 60%)
Flow Rate:1.0mL/min
Detection: UV/VIS　270nm
Temperature:40°Ｃ
Injection Volume:5µL
Title:Analysis　of　Catechins　ｂｙ　HPLC