Applications Database

T00907

 

Data code:

Analysis of isoflavones by HPLC

daidzin; glycitin; genistin; 6”-o-malonyldaidzin; 6”-o-malonylglycitin; 6”-o-acetyldaidzin; 6”-o-malonylgenistin; 6”-o-acetylglycitin; daidzein; glycitein; 6”-o-acetylgenistin; genistein

 
Chromatographic conditions
Column:TSKgelODS-100V 5μm 4.6×15, P/N 21461, 4.6mm ×15cm
Mobile Phase:A ; 0.1% CH3COOH in  (H2O/CH3CN=90/10)  B ; 0.1% CH3COOH in  (H2O/CH3CN=65/35) Gradient : 0min(B 0%)→50min(B 100%)
Flow Rate:1.0mL/min
Detection: UV/VIS 260nm
Temperature:40°C
Injection Volume:10µL
Title:Analysis of isoflavones by HPLC