Applications Database

T00928

 

Data code:

Analysis of phenolic antioxidants in butter by HPLC

PG; THBP; TBHQ; NDGA; BHA; OG; HMBP; DG; BHT

 
Chromatographic conditions
Column:TSKgelODS-100Z 5µm 4.6×15, P/N 21461, 4.6mm ×15cm
Mobile Phase:

A;5%CH3COOH B;CH3CN

Gradient:0min(B40%)→15min(B90%)→25min(B90%)

Flow Rate:1.0mL/min
Detection: FLD 275nm/365nm , UV/VIS 280nm 
Injection Volume:10µL
Title:Analysis of phenolic antioxidants in butter by HPLC