Applications Database

T00946

 

Data code:

Separation of Polyphenols

Vanillic acid; Syringic acid; p-Coumaric acid; Ferulic acid; Sinapic acid

 
Chromatographic conditions
Column:TSKgelODS-100Z 3μm 4.6mm×25cm, P/N 22747, 4.6mm×25cm
Mobile Phase:A;0.1%H3PO4 in H2O B;0.1%H3PO4 in CH3CN Gradient;0min(B10%)→10min(B15%)→30min(B15%)→31min(B100%)
Flow Rate:1.0mL/min
Detection: UV/VIS 280nm
Temperature:40°C
Injection Volume:20µL
Title:Separation of Polyphenols