Applications Database

T00949

 

Data code:

Analysis of polyphenols including in red wine by HILIC

quercetin; myricetin; rutin

 
Chromatographic conditions
Column:TSKgelAmide-80 3μm 4.6mm×15cm, P/N 21867, 4.6mm ×15cm
Mobile Phase:A;0.1%H3PO4 B;0.1%H3PO4 in CH3CN Gradient;0min(B95%)→15min(B50%)→20min(B50%)→21min(B95%)→30min(B95%)
Flow Rate:1.0mL/min
Detection: UV/VIS 360nm
Temperature:40°C
Injection Volume:5µL
Sample(s):wine
Title:Analysis of polyphenols including in red wine by HILIC