Applications Database

T01127

 

Data code:

Separation of Monoclonal Antibody in mouse ascites

monoclonal antibody

 
Chromatographic conditions
Column:TSKgel Chelate-5PW, P/N 08645, 7.5 mm ID × 7.5 cm
Mobile Phase:A: 20 mmol/L HEPES-MES-Acetate(pH 8.0)+ 0.5 mol/L NaCl;  B: 20 mmol/L HEPES-MES-Acetate (pH 4.0)+ 0.5 mol/L NaCl
Flow Rate:1.0 mL/min
Detection: UV/VIS 280 nm
Temperature:ambient
Title:Separation of Monoclonal Antibody in mouse ascites