Ca⁺⁺Pure-HA Hydroxyapatite Media from Tosoh Bioscience

July  17,  2018 -

King of Prussia, PA – Ca⁺⁺Pure-HA is a hydroxyapatite (Ca₁₀(PO₄)₆(OH)₂) chromatography media composed of calcium and phosphate and offers unique mixed mode properties for the purification of biomolecules. The formation of the Ca⁺⁺Pure-HA particle, both the ligand and the base bead, is created simultaneously from the same source of materials. Its highly selective nature often separates proteins otherwise shown to be homogeneous by electrophoresis and other chromatographic techniques. The highly selective and robust nature of Ca⁺⁺Pure-HA offers the flexibility to use this resin at any stage in a process from capture to final polishing.

While the selectivity and multiple modes of interaction of Ca⁺⁺Pure-HA media point to a seemingly limitless field of applications for its use, it is best suited for applications associated with the separation of biomolecules. Key applications for use of Ca⁺⁺Pure-HA include the purification of monoclonal and polyclonal antibodies, including IgG, IgA and IgM, IgG Fab regions, the purification of antibody fragments, the isolation of single-stranded from double-stranded DNA and the purification of fusion and phosphoproteins. 

Ca⁺⁺Pure-HA resin has been sintered at high temperatures for increased mechanical and pH stability, allowing it to withstand the rigors of industrial-scale applications. Ca⁺⁺Pure-HA has a demonstrated dynamic binding capacity (DBC), at 5% breakthrough, of 55 mg mAb/mg resin at 5 minute residence time. 

Ca⁺⁺Pure-HA media is effective for the removal of dimer and higher order aggregates from purified mAb samples. When Ca⁺⁺Pure-HA media is operated using potassium phosphate buffer as a loading buffer and potassium chloride as an elution buffer, the aggregate content is reduced from 6.6% to as low as 1.3%.

Tosoh Bioscience LLC has a staff of dedicated professionals to assist you with developing and scaling up Ca⁺⁺Pure-HA methods. Their knowledge of other Tosoh Bioscience LLC products and how they work together also enables them to help you integrate Ca⁺⁺Pure-HA into multistep processes to achieve the best overall results.