1. What is the support material for Tosoh Bioscience affinity resins?
TSKgel® affinity chromatography columns are based upon the G5000PW methacrylate porous resin. This “5PW” media is hydrophilic, with 100 nm pores and an estimated protein size exclusion limit of 1000 kDa.
2. What are the ligand concentrations, adsorption capacities and test analytes for the various Affinity column supplied by Tosoh Bioscience?
Characteristics of TSKgel® AFC columns:
Column packing
|
Ligand type
|
Ligand concentration
|
Adsorption capacity
|
Sample
|
| Boronate-5PW |
m-aminophenyl boronate |
not available |
40 µmol/mL - resin |
sorbitol |
| Chelate-5PW |
iminodiacetic acid |
20 µmol/mL - resin |
not available |
not available |
| Tresyl-5PW |
tresyl |
ca. 20 µmol/mL - resin |
>60 mg/g dry resin (coupling capacity) |
soybean trypsin inhibitor |
3. Can I get a TSKgel® Affinity LC column with non-metal wetted parts?
Yes. We supply glass columns in two formats: 5 mm ID × 5 cm and 8 mm ID × 7.5 cm with 10 µm pore size Pyrex frits.
Shipping solvents for TSKgel AFC columns:
Column
|
Shipping solvent
|
| Boronate-5PW |
distilled water |
| Chelate-5PW |
acetate buffer |
| Tresyl-5PW |
distilled water |
4. How do I load and desorb protein from a TSKgel® affinity column?
Specific instructions for retaining your protein as well as desorption procedures are included with each affinity column. Keep in the mind the analyte requires some structural feature that coincides with the retention mechanism of the affinity resin.
5. What endfitting do I use with my particular TSKgel® Affinity column?
Please visit the HPLC Columns Accessories section of the product catalog for the available fittings to be used with your TSKgel® column. Question #10 within the General FAQ category includes additional information on fittings and connectors.