Molar mass averages can be affected by changes in the environment and measuring conditions. Generally, these variations are the result of one or more factors including flow rate reproducibility, baseline drift and injection reproducibility. In addition to controlling column temperature, Tosoh engineers added temperature control for both pumps, the degasser chamber and inlet and outlet tubing on the EcoSEC Elite® GPC System to deliver top GPC analysis performance.
Figure 1 and Table 1 demonstrate the superiority of the EcoSEC Elite® GPC System for the determination of weight-average molar masses.
|
Vinyl chloride/vinyl acetate copolymer |
| Measurement date |
Mw |
CV(%) |
| Day 1 |
23165 |
0.305 |
| 2 |
23205 |
0.216 |
| 3 |
23029 |
0.109 |
| 4 |
23199 |
0.215 |
| 5 |
23091 |
0.182 |
| 6 |
23005 |
0.167 |
| AVG. |
23116 |
0.199 |
| RSD |
86.862 |
- |
| CV(%) |
0.376 |
- |
|
 |
| Intra day (n=10) |
CV: 0.199% |
| Inter day (n=6) |
CV: 0.376% |
|
Sample column:
Reference column:
Mobile phase:
Flow rate:
Detection:
Temperature:
Injection vol.:
|
TSKgel® SuperMultiporeHZ-M × 2
TSKgel® SuperH-RC
THF
0.35 mL/min, Sample and Reference
RI (EcoSEC® Elite GPC System)
40 °C
10 µL (1.0 g/L)
|
Figure 2 and Table 2 demonstrate the superiority of the EcoSEC Elite® GPC System for elution time reproducibility.
|
F-2 |
F-10 |
Measurement
date |
Retention
time (min) |
CV
(%) |
Retention
time (min) |
CV
(%) |
| Day 1 |
8.048 |
0.014 |
6.887 |
0.011 |
| 2 |
8.051 |
0.013 |
6.889 |
0.010 |
| 3 |
8.051 |
0.012 |
6.889 |
0.014 |
| 4 |
8.049 |
0.016 |
6.887 |
0.010 |
| 5 |
8.053 |
0.023 |
6.890 |
0.019 |
| 6 |
8.051 |
0.016 |
6.888 |
0.016 |
| AVG. |
8.050 |
0.016 |
6.888 |
0.013 |
| RSD |
0.002 |
- |
0.001 |
- |
| CV(%) |
0.022 |
- |
0.018 |
- |
|
 |
| Intra day (n=10) |
F2 CV: 0.016%; F10 CV: 0.013% |
| Inter day (n=6) |
F2 CV: 0.022%; F10 CV: 0.018% |
|
Sample column:
Reference column:
Mobile phase:
Flow rate:
Detection:
Temperature:
Injection vol.:
|
TSKgel® SuperMultiporeHZ-M × 2
TSKgel® SuperH-RC
THF
0.35 mL/min, Sample and Reference
RI (EcoSEC Elite® GPC System)
40 °C
10 µL (0.2 g/L)
|