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Hydrophobic Interaction Columns

TSKgel® Hydrophobic Interaction Chromatography Columns

The 2.5 μm non-porous methacrylate packing material of the TSKgel® Butyl-NPR (non-porous resin) columns is bonded with butyl groups. These columns are the best choice for high speed separations with excellent recovery, even for more hydrophobic samples. As in other modes of liquid chromatography, smaller particles provide higher efficiency. Non-porous resin provides higher resolution at higher flow rates as well as excellent mass recovery, allowing quantitation down to nanogram levels.

Of the three TSKgel® HIC columns, the TSKgel® Ether-5PW columns have intermediate hydrophobicity. TSKgel® Ether-5PW columns are stable in either acid or caustic cleaning regimens and provide excellent access to larger molecules with low diffusion coefficients.

The high porosity of TSKgel® Phenyl-5PW packings allows very large proteins to enter the internal pore structure, thereby maintaining high capacity for such compounds.

Particle Size (mean): 2.5 µm   
Pore Size (mean): non-porous                   Visit the TSKgel® Butyl-NPR product page.      
pH Range: 2.0 - 12.0
Functional Group: Butyl

Particle Size (mean): 10 µm   
Pore Size (mean): 100 nm                   Visit the TSKgel® Ether-5PW product page.      
pH Range: 2.0 - 12.0
Functional Group: Ether  

Particle Size (mean): 10, 13 and 20 µm   
Pore Size (mean): 100 nm                   Visit the TSKgel® Phenyl-5PW product page.      
pH Range: 2.0 - 12.0
Functional Group: Phenyl