TSKgel® SuperAW Columns for the Semi-Micro Analysis of Polar Organic-Soluble Polymers
TSKgel® SuperAW columns are used for high efficiency and high speed analysis of polymers that are soluble in polar organic solvents. Examples include cleansing gel in methanol, degree of saponification of polyvinylalcohol in HFIP, and sodium dodecylsulfate, polyimide, and cellulose derivatives in DMF with 10 mmol/L LiBr. TSKgel® SuperAW columns contain highly crosslinked hydroxylated polymethacrylate particles which minimize swelling in polar organic solvents.
For high throughput applications, the smaller dimensions of the TSKgel® SuperAW columns provide equivalent resolution in half the time and with minimal solvent waste compared to the TSKgel® Alpha columns.
Mixed bed TSKgel® SuperAW Column
The mixed bed column, TSKgel® SuperAWM-H, has an extended linear calibration range, and is suitable for samples with a broad molar mass (MM) distribution as well as samples with unknown molecular mass.
The following figure shows the polyethylene oxide, polyethylene glycol and ethylene glycol calibration curves of all TSKgel® SuperAW columns.
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Columns:
Mobile phase:
Flow rate:
Detection:
Samples: |
TSKgel® SuperAW columns, 6.0 mm ID × 15 cm
A. H2O
B. MeOH containing 10 mmol/L LiBr
C. DMF containing 10 mmol/L LiBr
0.6 mL/min
RI
polyethylene oxide (PEO), polyethylene glycol (PEG),
and ethylene glycol (EG) standards |
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Unlike TSKgel® PW-type columns, which are stable at most to a 50% organic mixed with water, TSKgel® SuperAW columns offer excellent stability in 100% aqueous to 100% organic solvents. As shown in the figure below, efficiency of all TSKgel® SuperAW columns is maintained when changing solvents from water via acetonitrile, DMF, DMSO, THF to HFIP.
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Columns:
Mobile phase:
Flow rate:
Detection:
Temperature:
Injection vol.:
Sample: |
TSKgel® SuperAW columns,
6.0 mm ID × 15 cm
H2O
0.6 mL/min
RI
25 °C
5 µL (2.5 g/L)
ethylene glycol |
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