TSKgel® PW Columns for Analysis of Water-Soluble Polymers
TSKgel® PW columns are composed of spherical, hydrophilic polymethacrylate beads. Particle sizes range from 12 µm for the smaller pore size columns to 17 µm for the larger pore size columns.
The main application areas for TSKgel® PW columns are the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides.
The figure below shows the calibration curves for all TSKgel® PW columns. Suitable solvents for various polymer samples are shown in the table below.
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Columns:
Mobile phase:
Flow rate:
Detection: |
TSKgel® PW columns,
all 7.5 mm ID × 60 cm
A. G2000PW, B. G2500PW,
C. G3000PW, D. G4000PW,
E. G5000PW, F. G6000PW,
G. GMPW
distilled H20
1.0 mL/min
RI
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Specialty TSKgel® PW Columns
The TSKgel® GMPW column is a mixed bed column containing a mixture of different pore sizes that has an extended linear calibration range, suitable for samples with a broad molar mass (MM) distribution, as well as unknown samples. A TSKgel® G6000PW column is available in PEEK hardware, TSKgel® BioAssist G6PW, when ultra-low sample adsorption is required, such as in virus analysis.
Recommended eluents for GFC of water-soluble polymers on TSKgel® PW columns
Type of Polymer
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Typical Sample
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Suitable Eluent
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Nonionic hydrophilic
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polyyethylene glycol
soluble starch, methyl cellulose,
pullulan dextran, hydroxyethyl cellulose
polyvinyl alcohol, polyacrylamide
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distilled water
0.01 mol/L NaOH
20% DMSO
Buffer or salt solution (e.g. 0.1-0.5 mol/L NaNO3)
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Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 mol/L NaNO3)
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Anionic Hydrophilic
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sodium chondroitin sulfate, sodium alginate, carboymethyl cellulose, sodium polyacrylate, sodium hyaluronate
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Buffer or salt solution (e.g. 0.1 mol/L NaNO3)
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Anionic Hydrophobic
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sulfonated lignin sodium salt,
sodium polystyrenesulfonate
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Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 mol/L NaNO3)
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Cationic Hydrophilic
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glycol chitosan, DEAE-dextran, poly(ethyleneimine), poly(trimethylaminoethyl methacrylate) iodide salt
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0.5 mol/L acetic acid with 0.3 mol/L Na2SO4 or 0.8 mol/L NaNO3
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Cationic Hydrophobic
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poly(4-vinylbenzyltrimethylammonium chloride), poly(N-methyl-2-vinylpyridinium) iodide salt
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0.5 mol/L acetic acid with 0.3 mol/L Na2SO4
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Amphoteric Hydrophilic
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peptides, proteins, poly- and oligosaccharides, DNA, RNA
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Buffer or salt solution (e.g. 0.1 mol/L NaNO3)
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Amphoteric hydrophobic
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blue dextran, collagen, gelatin, hydrophobic proteins, hydrophobic peptides
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Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 mol/L NaNO3 or 35-45% CH3CN in 0.1% TFA)
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