TOYOPEARL Butyl-650M

TOYOPEARL Butyl-650M is a hydrophobic interaction chromatography (HIC) resin for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with a Butyl ligand group. TOYOPEARL Butyl-650M resin has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates. TOYOPEARL Butyl-650M resin has a 100 nm pore size and a 65 µm bead size and is the second most hydrophobic of the TOYOPEARL HIC resins.

TOYOPEARL Butyl-650M resin can be used for high throughput capture, intermediate purification, and polishing process steps. The primary applications of this resin are for the separation of proteins, their isoforms, and aggregate removal. If greater resolution is needed, the smaller particle TOYOPEARL Butyl-650S resin may be used. If lower resolution is desired, the TOYOPEARL Butyl-650C can be used.

TOYOPEARL Butyl-650M resin has a dynamic binding capacity for lysozyme of 30 to 50 g/L. TOYOPEARL Butyl-650M resin maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0019802	TOYOPEARL Butyl-650M	25 mL	65	186	Inquire
0007477	TOYOPEARL Butyl-650M	100 mL	65	564	Inquire
0014702	TOYOPEARL Butyl-650M	1 L	65	-	Inquire
0007976	TOYOPEARL Butyl-650M	5 L	65	-	Inquire
0018355	TOYOPEARL Butyl-650M	50 L	65	-	Inquire

Product Attributes

Base bead:	HW-65
Pore size (mean):	100 nm
Particle size (mean):	65 µm
Ligand type:	Butyl
DBC:	30 - 50 g/L
Shipping buffer:	20% ethanol
pH stability:	1-13
Stability data:	10 years

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

Separation of Lipase Isozymes from Impurity

Resin:	TOYOPEARL Butyl-650M
Column size:	34 mm ID × 29 cm
Mobile phase:	linear gradient, 810 mL (NH₄)₂SO₄, 25% to 0% saturation in 0.01 mol/L acetate buffer, pH 5.6
Flow rate:	2 cm/hr
Detection:	UV @ 254 nm
Sample:	lipase from Geotrichum candidum
Sample load:	375 mg

For additional applications, please see our applications database.

Influence of Salt-type on Resolution

Chromatography on a TOYOPEARL Butyl-substituted support.

Resin:	TOYOPEARL Butyl-650M
Mobile phase:	4.1 mm ID × 4 cm
Mobile phase:	Buffer A: 20 mmol/L phosphate buffer in 1.0 mol/L indicated salt, pH 7.0 Buffer B: buffer A with 1.0 mol/L indicated salt
Flow rate:	484 cm/hr (1 mL/min)
Detection:	UV @ 280 nm

Fausnaugh, J.; Kennedy, L.; Regnier, F. J. Chromatography, 1984, 141, 317.

Benzyl Alcohol Removal

A 2% solution of benzyl alcohol in water has been identified as a suitable alternative to 20% ethanol as a preservative in resin storage solutions. A sample of TOYOPEARL Butyl-650M resin was prepared by adding 100 mL of aqueous 2% benzyl alcohol to 100 mL of suction filtered resin. A 100 mL aliquot of DI water was added to the filtered resin and stirred to make a slurry. This resin/ DI water slurry was allowed to stand for 5 minutes and was then suction filtered to remove the supernatant. This procedure was repeated 14 more times, for a total of 15 washes.

Samples of the filtered supernatant from the TOYOPEARL Butyl-650M resin was taken after the 5th, 10th, and 15th washes and analyzed for benzyl alcohol concentration. As demonstrated in the figure, a 2% benzyl alcohol solution can be removed from the TOYOPEARL Butyl-650M resin by thorough washing with DI water.

1.6 cm ID × 10 cm (20.1 mL)

Code #	Description	Literature Type
AN056	Clearance of 2% Benzyl Alcohol from TOYOPEARL Resins	Application Note
AN117	Simple and Effective Method for Purification of DMT-on Oligonucleotides using Hydrophobic Interaction Chromatography Resins	Application Note
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
IM14	Instruction Manual -- Principles of Hydrophobic Interaction Chromatography	Instruction Manual
PCAT03	TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic Interaction	Product Catalog
RIS0012	TOYOPEARL Butyl-650	Resin Information Sheet
TP231	An Effective One-step Purification of 5’-DMT-On Oligonucleotides Using Hydrophobic Interaction Chromatography	Technical Presentation

P/N	Description	Price ($)
OC41MDHIC-96	Resin Seeker TOYOPEARL HIC 96-well plate kit	-	Inquire
0045235	SkillPak HIC 1 mL column library	-	Inquire
0045233	SkillPak HIC 1 mL column library	284	Inquire
0045215	SkillPak TOYOPEARL Butyl-650M 1 mL columns (qty. 5)	284	Inquire
0045251	SkillPak TOYOPEARL Butyl-650M 5 mL column	235	Inquire
0019806	TOYOPEARL HICPAK	379	Inquire
0021376	ToyoScreen Butyl-650M, 1 mL (qty. 6)	-	Inquire
0021377	ToyoScreen Butyl-650M, 5 mL (qty. 6)	-	Inquire
0021398	ToyoScreen HIC Mix Pack, 1 mL (qty. 5)	-	Inquire
0021399	ToyoScreen HIC Mix Pack, 5 mL (qty. 5)	-	Inquire