Separation of Lipase Isozymes from Impurity
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Resin: |
TOYOPEARL Butyl-650M |
Column size: |
34 mm ID × 29 cm |
Mobile phase: |
linear gradient, 810 mL (NH4)2SO4, 25% to 0% saturation in 0.01 mol/L acetate buffer, pH 5.6 |
Flow rate: |
2 cm/hr |
Detection: |
UV @ 254 nm |
Sample: |
lipase from Geotrichum candidum |
Sample load: |
375 mg |
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