Dynamic Binding Capacity Comparision of HIC Resins
| Resins: |
A. TOYOPEARL Ether-650M
B. TOYOPEARL Phenyl-650M
C. TOYOPEARL Butyl-650M
D. TOYOPEARL Hexyl-650C
E. TOYOPEARL PPG-600M
F. TOYOPEARL SuperButyl-550C |
| Column size: |
7.8 mm ID × 20 cm |
| Mobile phase: |
1.8 mol/L sodium sulfate +
0.1 mol/L phosphate,pH 7.0
|
| Flow rate: |
100 cm/hr (0.8 mL/min)
|
| Detection: |
UV @ 280 nm |
| Temperature: |
ambient |
| Sample: |
1 g/L lysozyme |
| Sample load: |
as indicated in figure |
| Resins: |
binding capacity
(10% breakthrough) |
| TOYOPEARL Ether 650M |
12.5 g/L |
TOYOPEARL Phenyl 650M
|
27.5 g/L |
TOYOPEARL Butyl 650M
|
32.2 g/L |
TOYOPEARL Hexyl 650C
|
33.2 g/L |
TOYOPEARL PPG 600M
|
44.2 g/L |
TOYOPEARL SuperButyl 550C
|
54.3 g/L |
|
|
Selectivity Comparision of HIC -600M Resins
| Resins: |
A. TOYOPEARL PPG-600M
B. TOYOPEARL Phenyl-600M
C. TOYOPEARL Butyl-600M
|
| Column size: |
7.5 mm ID × 7.5 cm |
| Mobile phase: |
Buffer A: 1.8 mol/L (NH4)2SO4 + 0.1 mol/L sodium phosphate, pH 7.0
Buffer B: 0.1 mol/L sodium phosphate, pH 7.0
|
| Gradient: |
60 min linear gradient from buffer A to B
|
| Flow rate: |
136 cm/hr (1.0 mL/min)
|
| Detection: |
UV @ 280 nm |
| Temperature: |
ambient |
| Samples: |
1 g/L of: 1. ribonuclease A, 2. lysozyme, 3. α-chymotrypsinogen |
| Load volume: |
100 µL |
|