TOYOPEARL Hexyl-650C

TOYOPEARL Hexyl-650C is based on a 100 nm (1000 Å) pore size, 100 µm polymethacrylate base material bonded with C6 groups. This resin has the highest hydrophobicity of the TOYOPEARL HIC resins. It is used for very hydrophilic proteins or where a low salt elution environment is required. It is useful as an early capture step or for intermediate purification.

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0044465	TOYOPEARL Hexyl-650C	25 mL	100	206	Inquire
0019026	TOYOPEARL Hexyl-650C	100 mL	100	639	Inquire
0019027	TOYOPEARL Hexyl-650C	1 L	100	-	Inquire
0019028	TOYOPEARL Hexyl-650C	5 L	100	-	Inquire
0021973	TOYOPEARL Hexyl-650C	50 L	100	-	Inquire

Product Attributes

Base bead:	HW-65
Pore size (mean):	100 nm
Particle size (mean):	100 µm
Ligand type:	Hexyl
DBC:	30 - 50 g/L
Shipping buffer:	20% ethanol
pH stability:	1-13
Stability data:	10 years

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

Plasmid DNA Purification

Resin:	TOYOPEARL Hexyl-650C
Column size:	1.0 cm ID × 30 cm (23.6 mL)
Mobile phase:	Buffer A: 3.0 mol/L ammonium sulfate, 10 mmol/L Tris, 1 mmol/L EDTA, pH 7.4 Buffer B: 10 mmol/L Tris, 1 mmol/L EDTA, pH 7.4
Gradient:	3.0 mol/L ammonium sulfate to 1.0 mol/L ammonium sulfate (6 CV)
Flow rate:	153 cm/hr (2.0 mL/min)
Detection:	UV @ 254 nm
Samples:	Plasmid DNA in 3.0 mol/L ammonium sulfate

For additional applications, please see our applications database.

Dynamic Binding Capacity Comparision for HIC Resins

Lysozyme

HIC_Ether-650M_PPG-600M_SuperButyl-550C_Hexyl-650_Fig6.png

Resins:	binding capacity (10% breakthrough)
TOYOPEARL Ether 650M	12.5 g/L
TOYOPEARL Phenyl 650M	27.5 g/L
TOYOPEARL Butyl 650M	32.2 g/L
TOYOPEARL Hexyl 650C	33.2 g/L
TOYOPEARL PPG 600M	44.2 g/L
TOYOPEARL SuperButyl 550C	54.3 g/L

Resins:	A. TOYOPEARL Ether-650M B. TOYOPEARL Phenyl-650M C. TOYOPEARL Butyl-650M D. TOYOPEARL Hexyl-650C E. TOYOPEARL PPG-600M F. TOYOPEARL SuperButyl-550C
Column size:	7.8 cm ID × 20 cm
Mobile phase:	1.8 mol/L sodium sulfate + 0.1 mol/L phosphate,pH 7.0
Flow rate:	100 cm/hr (0.8 mL/min)
Detection:	UV @ 280 nm
Temperature:	ambient
Sample:	1 g/L lysozyme
Sample load:	as indicated in figure

Code #	Description	Literature Type
AN117	Simple and Effective Method for Purification of DMT-on Oligonucleotides using Hydrophobic Interaction Chromatography Resins	Application Note
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
IM14	Instruction Manual -- Principles of Hydrophobic Interaction Chromatography	Instruction Manual
PCAT03	TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic Interaction	Product Catalog
PD05	Optimizing Pore Size to Improve Throughput in Downstream Purification of Biological Therapeutic Products	Performance Data
RIS0014	TOYOPEARL Hexyl-650C	Resin Information Sheet
TP231	An Effective One-step Purification of 5’-DMT-On Oligonucleotides Using Hydrophobic Interaction Chromatography	Technical Presentation

P/N	Description	Price ($)
OC41MDHIC-96	Resin Seeker TOYOPEARL HIC 96-well plate kit	650	Inquire
0045237	SkillPak HIC 1 mL column library	-	Inquire
0045238	SkillPak HIC 1 mL column library	-	Inquire
0045234	SkillPak HIC 1 mL column library	-	Inquire
0045233	SkillPak HIC 1 mL column library	293	Inquire
0045219	SkillPak TOYOPEARL Hexyl-650C 1 mL columns (qty. 5)	293	Inquire
0045255	SkillPak TOYOPEARL Hexyl-650C 5 mL column	242	Inquire
0043125	TOYOPEARL HICPAK-C	586	Inquire
0021378	ToyoScreen Hexyl-650C, 1 mL (qty. 6)	-	Inquire
0021379	ToyoScreen Hexyl-650C, 5 mL (qty. 6)	-	Inquire
0021398	ToyoScreen HIC Mix Pack, 1 mL (qty. 5)	-	Inquire
0021399	ToyoScreen HIC Mix Pack, 5 mL (qty. 5)	-	Inquire