TSKgel Phenyl-5PW (20) resin is a hydrophobic interaction chromatography resin for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with a Phenyl ligand group. TSKgel Phenyl-5PW (20) is more hydrophobic than the Ether-5PW resins and is the best choice for the separation of moderately hydrophobic proteins or monoclonal antibodies. This resin has a 100 nm pore size and a 20 µm bead size.

TSKgel Phenyl-5PW (20) resin has a high chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates.  TSKgel PW resins vary from Tosoh’s TOYOPEARL resins by having a higher degree of crosslinking which is necessitated by the higher flow pressures needed when using smaller particles for chromatography. The increased level of crosslinking decreases the number of sites available for ligand immobilization when producing TSKgel PW hydrophobic interaction chromatography resins.

A TSKgel PW resin will have a lower dynamic binding capacity than the corresponding TOYOPEARL material however; chromatographic efficiency, peak width, and resolution are improved because of their smaller particle sizes. In this way they are higher performing chromatography resins than their TOYOPEARL counterparts.

TSKgel Phenyl-5PW (20) resin is best used for high resolution process polishing steps. If a product with less resolution is needed, the larger particle TSKgel Phenyl-5PW (30) or TOYOPEARL Phenyl-650 resins may be used.

Because similarly functionalized TSKgel PW and TOYOPEARL resin types have the same backbone polymer chemistry, the selectivity for proteins and impurities remains the same.