TOYOPEARL Sulfate-650F

TOYOPEARL Sulfate-650F resin is a strong cation exchange resin that exhibits high salt tolerance. This resin offers the strongest capture of monoclonal antibody (mAb) aggregates over a wide pH range without losing its binding capacity for mAb. With the use of optimized binding conditions, a dynamic binding capacity of > 120 g/L of mAb can be easily achieved with TOYOPEARL Sulfate-650F. This high binding capacity translates into lower operating costs per gram of antibody produced.

A TOYOPEARL HW-65F polymeric bead has been functionalized with a sulfate (SO^4-) group. The 100 nm pore size of this resin, along with proprietary bonding technology, makes TOYOPEARL Sulfate-650F ideal for applications performed in physiological conditions or for post-protein A removal of aggregates.

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0023467	TOYOPEARL Sulfate-650F	100 mL	45	590	Inquire
0023468	TOYOPEARL Sulfate-650F	250 mL	45	1,105	Inquire
0023469	TOYOPEARL Sulfate-650F	1 L	45	-	Inquire
0023470	TOYOPEARL Sulfate-650F	5 L	45	-	Inquire
0023471	TOYOPEARL Sulfate-650F	50 L	45	-	Inquire

Product Attributes

Base bead:	HW-65
Pore size (mean):	100 nm
Particle size (mean):	45 µm
Ligand type:	strong cation
DBC:	>120 g/L
Shipping buffer:	20% (v/v) ethanol containing 0.2 mol/L sodium acetate
pH stability:	2-13

Structure:

Aggregate Removal

Resin:	TOYOPEARL Sulfate-650F
Column size:	6.6 mm ID × 20 cm (6.8 mL)
Mobile phase:	A: 0.1 mol/L sodium acetate, pH 5.0 B: mobile phase A + 1 mol/L NaCl
Gradient:	0-50% B, 20 CV 100% B, 5 CV
Flow rate:	300 cm/hr (4 min residence time)
Detection:	UV @ 280 nm (mAU), conductivity (mS/cm)
Temperature:	ambient
Injection vol.:	6.8 mL (10 mg/mL-resin load ratio)
Sample:	IgG, protein A-purified, aggregated, 10 g/L
Instrument:	ÄKTA avant 25

For additional applications, please see our applications database.

Wide Working Range of pH: Flexibility

TOYOPEARL Sulfate-650F resin works with a wide pH range

High Resolution and High Salt Tolerance

Greater resolution between monomer and aggregates
Highest salt tolerance versus other Fractogel resin on the market

Code #	Description	Literature Type
AN098	Increase Monoclonal Antibody Purity With TOYOPEARL Sulfate-650F Resin: a Strong Cation Exchange Resin for Capture and Removal of mAb Aggregates	Application Note
BR10	Best-In-Class Bioprocessing Resins	Brochure
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
PO45	TOYOPEARL Sulfate-650F	Product Overview
RIS0037	TOYOPEARL Sulfate-650F	Resin Information Sheet
TP217	Evaluation of Viral Clearance for Monoclonal Antibody Chromatographic Process Steps	Technical Presentation
TP229	Two-Step Purification Process for Mouse Monoclonal Antibody from Hybridoma Cell Line	Technical Presentation

P/N	Description	Price ($)
OC41MDSLFT-650F	Resin Seeker TOYOPEARL Sulfate-650F 96-well plate kit	-	Inquire
0045228	SkillPak Antibody 1 mL column library	434	Inquire
0045232	SkillPak Best in Class 1 mL column library	430	Inquire
0045266	SkillPak Best in Class 5 mL column library	1,406	Inquire
0045227	SkillPak CEX 1 mL column library	341	Inquire
0045229	SkillPak mAb Platform 1 mL column library	430	Inquire
0045263	SkillPak mAb Platform 5 mL column library	844	Inquire
0045230	SkillPak Salt Tolerant 1 mL column library	341	Inquire
0045264	SkillPak Salt Tolerant 5 mL column library	469	Inquire
0045205	SkillPak TOYOPEARL Sulfate-650F 1 mL columns (qty. 5)	284	Inquire
0045241	SkillPak TOYOPEARL Sulfate-650F 5 mL column	235	Inquire
0045027	ToyoScreen RoboColumns Sulfate-650F, 200 µL	-	Inquire
0045028	ToyoScreen RoboColumns Sulfate-650F, 600 µL	309	Inquire
0023472	ToyoScreen Sulfate-650F, 1 mL (qty. 6)	-	Inquire
0023473	ToyoScreen Sulfate-650F, 5 mL (qty. 6)	-	Inquire