TSKgel UP-SW3000

TSKgel UP-SW3000 columns are designed for the analysis of monoclonal antibodies and other biopharma products and can be used on both HPLC and UHPLC systems. The columns are packed with 2 μm silica-based beads shielded with a hydrophilic diol-type bonded phase that prevents the silica surface from interacting with protein samples.

TSKgel UP-SW3000 columns feature the same pore size as the well-established TSKgel G3000SWxl columns. Hence methods developed using TSKgel G3000SWxl columns can easily be transferred to TSKgel UP-SW3000 columns on conventional HPLC systems as well as on UHPLC systems.

TSKgel UP-SW3000 columns offer higher resolution, improved peak shape and increased efficiency yielding methods that are robust, reproducible and easily transferrable between UHPLC and HPLC systems.

TSKgel UP-SW3000 columns offer superior reproducibility injection-to-injection, from column-to-column within the same lot and from lot-to-lot. %RSD values for peak parameters including retention times, area peak height, peak asymmetry, and theoretical plate count demonstrate the exceptional reproducibility of these columns.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0023449	TSKgel UP-SW3000	2	Stainless Steel	4.6	15	2,097	Inquire
0023448	TSKgel UP-SW3000	2	Stainless Steel	4.6	30	2,825	Inquire

Product Attributes

Base material:	silica
Particle size (mean):	2 µm
Pore size (mean):	25 nm
Functional group:	Diol
pH stability:	2.5-7.5
Calibration range (proteins):	10 - 500 kDa

Protein Calibration Curves

TSKgel UP-SW calibration curves

Columns:	A. TSKgel UP-SW2000, 2 µm, 4.6 mm ID × 30 cm B. TSKgel UP-SW3000, 2 µm, 4.6 mm ID × 30 cm C. TSKgel UP-SW Aggregate, 3 µm, 4.6 mm ID × 30 cm
Mobile phase:	100 mmol/L phosphate buffer, pH 6.7, + 100 mmol/L Na₂SO₄ + 0.05% NaN₃
Flow rate:	0.35 mL/min
Detection:	UV @ 280 nm
Temperature:	25 °C
Injection vol.:	10 µL
Samples:	1. thyroglobulin (MW 640,000 Da) (1' thyroglobulin dimer) 2. γ-globulin (MW 155,000 Da) (2' γ-globulin dimer) 3. ovalbumin (MW 47,000 Da) 4. riboneclease A (MW 13,000 Da) 5. p-aminobenzoic acid (MW 137 Da)

mAb Aggregate Analysis

Columns:	TSKgel UP-SW3000, 2 µm, 4.6 mm ID × 15 cm
Mobile phase:	100 mmol/L sodium phosphate buffer, pH 6.8 + 100 mmol/L sodium sulfate + 0.05% sodium azide
Gradient:	isocratic (0-7 min)
Flow rate:	0.35 mL/min
LC system:	Ultimate^® 3000RS UHPLC system
Detection:	UV @ 280 nm
Temperature:	25 °C
Injection vol.:	10 µL
Sample:	mAb (0.4 mg/mL)

Fast Analysis of mAb Sample

Columns:	TSKgel UP-SW3000, 2 µm, 4.6 mm ID × 15 cm
Mobile phase:	100 mmol/L sodium phosphate buffer, pH 6.8 + 100 mmol/L sodium sulfate + 0.05% sodium azide
Gradient:	isocratic (0-4 min)
Flow rate:	0.5 mL/min
LC system:	Ultimate^® 3000RS UHPLC system
Detection:	UV @ 280 nm
Temperature:	25 °C
Injection vol.:	10 µL
Sample:	mAb (0.4 mg/mL)

Comparison of QC Standard Protein Mixture

Columns:	TSKgel UP-SW3000, 2 µm, 4.6 mm ID × 15 cm Competitor column, 1.7 µm, 4.6 mm ID × 15 cm
Mobile phase:	100 mmol/L sodium phosphate buffer, pH 6.7 + 100 mmol/L Na₂SO₄ + 0.05% NaN₃
Gradient:	isocratic
Flow rate:	0.35 mL/min
Detection:	UV @ 280 nm
Temperature:	25 °C
Injection vol.:	5 µL
Sample:	QC standard protein test mixture: thyroglobulin, 640 kDa, 0.5 g/L γ-globulin, 155 kDa, 1 g/L ovalbumin, 47 kDa, 1 g/L ribonuclease A, 13,700 Da, 1.5 g/L p-aminobenzoic acid, 137 Da, 0.01 g/L

Comparison of mAb Analysis

Columns:	TSKgel UP-SW3000, 2 µm, 4.6 mm ID × 30 cm Competitor column, 1.7 µm, 4.6 mm ID × 15 cm
Mobile phase:	100 mmol/L sodium phosphate buffer, pH 6.7 + 100 mmol/L Na₂SO₄ + 0.05% NaN₃
Gradient:	isocratic
Flow rate:	0.35 mL/min
Detection:	UV @ 280 nm
Temperature:	25 °C
Sample:	mAb

For additional applications, please see our applications database.

Code #	Description	Literature Type
AN088	Increased Monoclonal Antibody Resolution with TSKgel UP-SW3000 Columns	Application Note
AN089	Increased Resolution and Reproducibility with TSKgel UP-SW3000 Columns Versus a Competitive SEC Column	Application Note
AN090	Improving Resolution and Sample Throughput for Protein Analysis using TSKgel UP-SW3000 Columns	Application Note
AN095	Rapid and accurate therapeutic mAb aggregate analysis using a TSKgel UP-SW3000, 2μm, SEC Column	Application Note
AN097	The Usage of Isopropyl Alcohol in Size Exclusion Chromatography for Monoclonal Antibody Separation	Application Note
AN101	SEC/MS Analysis of a Bispecific Antibody	Application Note
AN105	Comparison of HPLC and UHPLC Methods in the Quality Control of mAb Separations by SEC	Application Note
AN106	UHPLC Analysis of Biosimilars Using Size Exclusion Chromatography	Application Note
AN109	U/HPLC Workflow Solution for Biosimilar Characterization	Application Note
AN127	Characterization of Monoclonal Antibodies Using Native SEC-MS and Its Comparison to Denaturing RP-MS Technique	Application Note
BR09	Solutions for Monoclonal Antibody Analysis and Characterization	Brochure
DS1241	TSKgel UP-SW3000 Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
PD17	Superior Reproducibility of TSKgel UP-SW3000 Columns	Performance Data
PO43	TSKgel UP-SW3000, 2 μm UHPLC/HPLC SEC Columns	Product Overview
TP214	Characterization of New 2 μm Particle Size, 25 nm Pore Size Analytical Size Exclusion Chromatography Column With Larger Exclusion Limit	Technical Presentation
TP215	Mass Spectrometric Characterization of Intact Half-mAb separated from intact monoclonal antibody and fragments using analytical size exclusion chromatography column	Technical Presentation
TP216	Analysis of Bispecific mAb by Size Exclusion Chromatography and Mass Spectrometry	Technical Presentation
TP219	Comparison of HPLC and UHPLC Methods to Optimize Monoclonal Antibody Separation by Size Exclusion Chromatography	Technical Presentation
TP221	Analytical Characterization of a Biosimilar Using a 2 μm Silica Based Size Exclusion Chromatography Column	Technical Presentation
TP222	Separation of Biosimilar Using a 2 μm Silica Based Size Exclusion Chromatography Column	Technical Presentation
TP224	Workflow Solution for the Characterization of Biosimilar using Different Modes of Analytical Chromatography Techniques	Technical Presentation
TP229	Two-Step Purification Process for Mouse Monoclonal Antibody from Hybridoma Cell Line	Technical Presentation
TP232	Comparison of Denaturing RP-ESI-MS and Native SEC-ESI-MS for the Characterization of Monoclonal Antibodies	Technical Presentation

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0023451	TSKgel UP-SW3000 DC Guard Column for 4.6 mm ID column	2	Stainless Steel	4.6	2	824
0023450	TSKgel UP-SW3000 Guard Column for 4.6 mm ID column	2	Stainless Steel	4.6	2	824