TOYOPEARL Ether-650M

TOYOPEARL Ether-650M is a hydrophobic interaction chromatography (HIC) resin for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with an Ether ligand group. TOYOPEARL Ether-650M resin has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates. TOYOPEARL Ether-650M resin has a 100 nm pore size and a 65 µm bead size and is the least hydrophobic of the TOYOPEARL HIC resins.

TOYOPEARL Ether-650M resin can be used for high throughput capture, intermediate purification, and polishing process steps. If greater resolution is needed, the smaller particle TOYOPEARL Ether-650S or TSKgel Ether-5PW resins may be used. As both the TOYOPEARL Ether-650 and TSKgel Ether-5PW resins use the same ligand and backbone chemistry, the selectivity between the two resin types will be unchanged.

TOYOPEARL Ether-650M resin has a dynamic binding capacity for lysozyme of 10 to 30 g/L. TOYOPEARL Ether-650M resin maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0019805	TOYOPEARL Ether-650M	25 mL	65	201	Inquire
0016173	TOYOPEARL Ether-650M	100 mL	65	623	Inquire
0016175	TOYOPEARL Ether-650M	1 L	65	-	Inquire
0016177	TOYOPEARL Ether-650M	5 L	65	-	Inquire

Product Attributes

Base bead:	HW-65
Pore size (mean):	100 nm
Particle size (mean):	65 µm
Ligand type:	Ether
DBC:	10 - 30 g/L
Shipping buffer:	20% ethanol
pH stability:	1-13
Stability data:	10 years

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

Purification of Monoclonal Antibodies

Resins:	A. TSKgel Ether-5PW (prepacked HPLC column) B. TOYOPEARL Ether-650M
Column size:	7.5 mm ID × 7.5 cm
Mobile phase:	Buffer A: 1.5 mol/L (NH₄)₂SO₄+ 0.1 mol/L phosphate, pH 7.0 Buffer B: 0.1 mol/L phosphate, pH 7.0
Gradient:	60 min linear gradient from buffer A to B
Flow rate:	136 cm/hr (1.0 mL/min)
Detection:	UV @ 280 nm
Temperature:	ambient
Samples:	A: 1.5 mg/100 µL anti-chicken 14 kDa lectin B: 0.76 mg/50 µL diluted ascites fluid
Load volume:	150µL

For additional applications, please see our applications database.

Dynamic Binding Capacity Comparision for HIC Resins

Lysozyme

HIC_Ether-650M_PPG-600M_SuperButyl-550C_Hexyl-650_Fig6.png

Resins:	binding capacity (10% breakthrough)
TOYOPEARL Ether 650M	12.5 g/L
TOYOPEARL Phenyl 650M	27.5 g/L
TOYOPEARL Butyl 650M	32.2 g/L
TOYOPEARL Hexyl 650C	33.2 g/L
TOYOPEARL PPG 600M	44.2 g/L
TOYOPEARL SuperButyl 550C	54.3 g/L

Resins:	A. TOYOPEARL Ether-650M B. TOYOPEARL Phenyl-650M C. TOYOPEARL Butyl-650M D. TOYOPEARL Hexyl-650C E. TOYOPEARL PPG-600M F. TOYOPEARL SuperButyl-550C
Column size:	7.8 cm ID × 20 cm
Mobile phase:	1.8 mol/L sodium sulfate + 0.1 mol/L phosphate, pH 7.0
Flow rate:	100 cm/hr (0.8 mL/min)
Detection:	UV @ 280 nm
Temperature:	ambient
Sample:	1 g/L lysozyme
Sample load:	as indicated in figure

Code #	Description	Literature Type
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
IM14	Instruction Manual -- Principles of Hydrophobic Interaction Chromatography	Instruction Manual
PCAT03	TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic Interaction	Product Catalog
PD05	Optimizing Pore Size to Improve Throughput in Downstream Purification of Biological Therapeutic Products	Performance Data
RIS0013	TOYOPEARL Ether-650	Resin Information Sheet

P/N	Description	Price ($)
OC41MDHIC-96	Resin Seeker TOYOPEARL HIC 96-well plate kit	650	Inquire
0045234	SkillPak HIC 1 mL column library	-	Inquire
0045233	SkillPak HIC 1 mL column library	284	Inquire
0045220	SkillPak TOYOPEARL Ether-650M 1 mL columns (qty. 5)	284	Inquire
0045256	SkillPak TOYOPEARL Ether-650M 5 mL column	235	Inquire
0019806	TOYOPEARL HICPAK	379	Inquire
0021372	ToyoScreen Ether-650M, 1 mL (qty. 6)	-	Inquire
0021373	ToyoScreen Ether-650M, 5 mL (qty. 6)	-	Inquire