TOYOPEARL Phenyl-600M

TOYOPEARL Phenyl-600M is based on a 75 nm (750 Å) pore size, 65 µm polymethacrylate base material bonded with phenyl groups. Its pore size has been optimized for the purification of monoclonal antibodies. It has an intermediate hydrophobicity, which is slightly higher than the one of the 100 nm pore size TOYOPEARL Phenyl-650.

TOYOPEARL Phenyl-600M is typically used for intermediate purification or polishing steps. It is a very high recovery and high dynamic capacity resin for both large and small proteins. When used as the polishing step up to an extra 5% yield may be seen compared to other Phenyl resins.

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0021887	TOYOPEARL Phenyl-600M	25 mL	65	213	Inquire
0021888	TOYOPEARL Phenyl-600M	100 mL	65	666	Inquire
0021889	TOYOPEARL Phenyl-600M	1 L	65	-	Inquire
0021890	TOYOPEARL Phenyl-600M	5 L	65	-	Inquire
0021891	TOYOPEARL Phenyl-600M	50 L	65	-	Inquire

Product Attributes

Base bead:	HW-60
Pore size (mean):	75 nm
Particle size (mean):	65 µm
Ligand type:	Phenyl
DBC:	45 - 65 g/L
Shipping buffer:	20% ethanol
pH stability:	1-13
Stability data:	10 years

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

Dynamic Binding Capacity of Polyclonal IgG on Various HIC Resins

Resins:	A. TOYOPEARL PPG-600M B. TOYOPEARL Phenyl-650M C. TOYOPEARL Butyl-650M D. Competitor Phenyl Agarose E. TOYOPEARL Phenyl-600M
Column size:	7.8 mm ID × 20 cm
Mobile phase:	0.1 mol/L sodium phosphate, pH 7.0 + 0.8 mol/L (NH₄)₂SO₄
Flow rate:	300 cm/hr (2.4 mL/min)
Detection:	UV @ 280 nm
Temperature:	25 °C
Sample:	1.0 g/L polyclonal IgG

DBC was calculated at 10% breakthrough

Selectivity Comparison of Phenyl-type Resins

Resins:	A. TOYOPEARL Phenyl-600M B. TOYOPEARL Phenyl-650M C. Competitor Phenyl Agarose
Column size:	7.5 mm ID × 7.5 cm
Mobile phase:	Buffer A: 1.8 mol/L (NH₄)₂SO₄+ 0.1 mol/L sodium phosphate, pH 7.0 Buffer B: 0.1 mol/L sodium phosphate, pH 7.0
Gradient:	60 min linear gradient from buffer A to B
Flow rate:	136 cm/hr (1.0 mL/min)
Detection:	UV @ 280 nm
Temperature:	ambient
Sample:	1.0 g/L of: 1. ribonuclease A, 2. lysozyme
Load volume:	100 µL

Code #	Description	Literature Type
AN059	Hydrophobic Interaction Chromatography: Effects of Mixed Electrolytes on Protein Separations	Application Note
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
IM14	Instruction Manual -- Principles of Hydrophobic Interaction Chromatography	Instruction Manual
PCAT03	TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic Interaction	Product Catalog
PO18	TOYOPEARL Phenyl-600M for high capacity Hydrophobic Interaction Chromatography and high recovery of large and small proteins	Product Overview
RIS0045	TOYOPEARL Phenyl-600M	Resin Information Sheet
TP124	Novel TOYOPEARL Hydrophobic Interaction Chromatographic Resin for Antibody Purification	Technical Presentation

P/N	Description	Price ($)
OC41MDHIC-96	Resin Seeker TOYOPEARL HIC 96-well plate kit	650	Inquire
0045234	SkillPak HIC 1 mL column library	-	Inquire
0045238	SkillPak HIC 1 mL column library	-	Inquire
0045236	SkillPak HIC 1 mL column library	341	Inquire
0045216	SkillPak TOYOPEARL Phenyl-600M 1 mL columns (qty. 5)	284	Inquire
0045252	SkillPak TOYOPEARL Phenyl-600M 5 mL column	235	Inquire
0021398	ToyoScreen HIC Mix Pack, 1 mL (qty. 5)	-	Inquire
0021399	ToyoScreen HIC Mix Pack, 5 mL (qty. 5)	-	Inquire
0021892	ToyoScreen Phenyl-600M, 1 mL (qty. 6)	-	Inquire
0021893	ToyoScreen Phenyl-600M, 5 mL (qty. 6)	-	Inquire
0045031	ToyoScreen RoboColumns Phenyl-600M, 200 µL	-	Inquire
0045032	ToyoScreen RoboColumns Phenyl-600M, 600 µL	-	Inquire